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http://dx.doi.org/10.5850/JKSCT.2013.37.1.17

Analysis of the Pigment Contents of Commercial Indigo Powders and Their Effect on the Color and the Antimicrobial Function of Dyed Cotton Fabrics  

Oh, Jeeeun (Dept. of Fashion Industry, University of Incheon)
Ahn, Cheunsoon (Dept. of Fashion Industry, University of Incheon)
Publication Information
Journal of the Korean Society of Clothing and Textiles / v.37, no.1, 2013 , pp. 17-26 More about this Journal
Abstract
Market available fermented indigo powders of Indian origin (FI1, FI2), Chinese origin (FC1, FC2), and raw indigo powders of Indian origin (R1, R2) were examined using TLC and HPLC analyses to investigate their pigment contents. TLC analysis gave $R_f$ values of 0.81 and 0.72 for blue and red pigments, respectively. All the powder products and the synthetic and natural indigo standards eluted at 6.9 min and 8.3 min in the HPLC chromatograms, and the peaks showed the ${\lambda}_{max}$ at 610nm and 542nm, representing indigotin and indirubin, respectively. The pigment content calculated based on the area of indigotin and indirubin peaks in the HPLC chromatograms showed that the indigotin content was higher in FC1 and FC2, while FI1 and FI2 had a higher indirubin content. The relative percentage of indirubin was the highest in R2, but the HPLC peak intensity was quite low. Despite the higher indigotin content in FC1 and FC2, cotton dyed with FI1 and FI2 (versus cotton dyed with FC1 and FC2) showed a higher blue (B) hue, the highest K/S values, and the highest antimicrobial effect.
Keywords
Indigo powder; Indigotin; Indirubin; HPLC analysis; Antimicrobial effect;
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1 Kunikata, T., Tatefuji, T., Aga, H., Iwaki, K., Ikeda, M., & Kurimoto, M. (2000). Indirubin inhibits inflammatory reactions in delayed-type hypersensitivity. European Journal of Pharmacology, 410(1), 93−100.   DOI   ScienceOn
2 Laitonjam, W. S., & Wangkheirakpam, S. D. (2011). Comparative study of the major components of the indigo dye obtained from Strobilanthes flaccidifolius Nees. and Indigofera tinctoria Linn. International Journal of Plant Physiology and Biochemistry, 3(7), 108−116.
3 Liau, B. C., Jong, T. T., Lee, M. R., & Chen, S. S. (2007). LCAPCI- MS method for detection and analysis of tryptanthrin, indigo, and indirubin in Danqingye and Banlangen. Journal of Pharmaceutical and Biomedical Analysis, 43 (1), 346−351.
4 Maier, W., Schumann, B., & Groger, D. (1990). Biosynthesis of indoxyl derivatives in Isatis tinctoria and Polygonum tinctorium. Phytochemistry, 29(3), 817−819.   DOI   ScienceOn
5 Minami, Y., Kanafuji, T., & Miura, K. (1996). Purification and characterization of a $\beta$-glucosidase from Polygonum tinctorium, which catalyzes preferentially the hydrolysis of indican. Bioscience, Biotechnology, and Biochemistry, 60(1), 147−149.
6 Minami, Y., Nishimura, O., Hara-Nishimura, I., Nishimura, M., & Matsubara, H. (2000). Tissue and intracellular localization of indican and the purification and characterization of indican synthase from indigo plants. Plant and Cell Physiology, 41(2), 218−225.   DOI   ScienceOn
7 Minami, Y., Takao, H., Kanafuji, T., Miura, K., Kondo, M., Hara-Nishimura, I., Nishimura, M., & Matsubara, H. (1997). $\beta$-Glucosidase in the indigo plant: Intracellular localization and tissue specific expression in leaves. Plant and Cell Physiology, 38(9), 1069−1074.   DOI   ScienceOn
8 Oberthur, C., Graf, H., & Hamburger, M. (2004a). The content of indigo precursors in Isatis tinctoria leaves-a comparative study of selected accessions and post-harvest treatments. Phytochemistry, 65(24), 3261−3268.   DOI   ScienceOn
9 Oberthur, C., Schneider, B., Graf, H., & Hamburger, M. (2004b). The elusive indigo precursors in woad (Isatis tinctoria L.) -Identification of the major indigo precursor, Isatin A, and a structure revision of Isatin B. Chemistry & Biodiversity, 1(1), 174−182.   DOI   ScienceOn
10 Oh, J., & Ahn, C. (2011). A study on the current status and dyeing characteristics of natural indigo powder dye. Journal of the Korean Society of Clothing and Textiles, 35(7), 736−747.   DOI   ScienceOn
11 Puchalska, M., Polec-Pawlak, K., Zadrozna, I., Hryszko, H., & Jarosz, M. (2004). Identification of indigoid dyes in natural organic pigments used in historical art objects by high-performance liquid chromatography coupled to electrospray ionization mass spectrometry. Journal of Mass Spectrometry, 39(12), 1441−1449.   DOI   ScienceOn
12 Shin, J. H., & Lee, H. J. (1993). Cultural conditions and growth characteristics of indigo (Polygonum tinctorium) cells in an air-lift bioreactor. Korean Journal of Biotechnology and Bioengineering, 8(3), 193−199.
13 Shin, Y., Son, K., & Yoo, D. I. (2009). Dyeing properties and storage stability of leaf powder prepared from Dyer's Knotweed (I)-By freeze drying method-. Journal of the Korean Society of Dyers and Finishers, 21(1), 10−20.
14 Stoker, K. G., Cooke, D. T., & Hill, D. J. (1998). An improved method for the large scale processing of woad (Isatis tinctoria) for possible commercial production of woad indigo. Journal of Agricultural Engineering Research, 71 (4), 315−320.
15 Clark, R. J. H., Cooksey, C. J., Daniels, M. A. M., & Withnall, R. (1993). Indigo, woad, and tyrian purple: Important vat dyes from antiquity to the present. Endeavour, 17(4), 191−199.   DOI   ScienceOn
16 Strobel, J., & Groger, D. (1989). Uber das vorkommen von indigovorstufen in Isatis-species [Indigo precursors of isatis species]. Biochmie und Physiologie der Pflanzen, 184 (3-4), 321−327.   DOI
17 Torimoto, N. (1987). An indigo plant as a teaching material. Journal of Chemical Education, 64(4), 332−334.   DOI
18 Angelini, L. G., Tozzi, S., & Di Nasso, N. N. O. (2004). Envi-ronmental factors affecting productivity, indican content, and indigo yield in Polygonum tinctorium Ait., a subtropical crop grown under temperate conditions. Journal of Agricultural and Food Chemistry, 52(25), 7541−7547.   DOI   ScienceOn
19 Chung, I. M., Kim, I. H., & Nam, S. W. (1998). Structural analysis of natural indigo colorants extracted from polygonum tinctorium. Journal of the Korean Society of Dyers and Finishers, 10(3), 20−28.
20 Chung, I. M., & Woo, S. O. (2002). Effect of reducing agent, sodium hydrosulfite on the natural indigo dyeing of silk fabric. Korean Journal of Sericultural Science, 44(2), 93− 98.
21 Cooksey, C. J. (2007). Indigo: An annotated bibliography. Biotechnic and Histochemistry, 82(2), 105−125.   DOI   ScienceOn
22 Gilbert, K. G., Maule, H. G., Rudolph, B., Lewis, M., Vandenburg, H., Sales, E., Tozzi, S. & Cooke, D. T. (2004). Quantitative analysis of indigo and indigo precursors in leaves of Isatis spp. and Polygonum tinctorium. Biotechnology Progress, 20(4), 1289−1292.   DOI   ScienceOn
23 Hamburger, M. (2002). Isatis tinctoria: From the rediscovery of an ancient medicinal plant towards a novel anti-inflammatory phytopharmaceutical. Phytochemistry Reviews, 1(3), 333−344.   DOI
24 Hoessel, R., Leclerc, S., Endicott, J., Nobel, M. E. M., Lawrie, A., Tunnah, P., Leost, M., Damiens, E., Marie, D., Marko, D., Niederberger, E., Tang, W., Eisenbrand, G., & Meijer, L. (1999). Indirubin, the active constituent of a Chinese antileukaemia medicine, inhibits cyclin-dependent kinases. Nature Cell Biology, 1, 60−67.   DOI   ScienceOn
25 Kang, J. Y., & Ryu, H. S. (2001). Dyeability of saxon blue derived from Polygonum tinctorium. Journal of the Korean Society of Clothing and Textiles, 25(10), 1763−1769.
26 Kokubun, T., Edmonds, J., & John, P. (1998). Indoxyl derivatives in woad in relation to medieval indigo production. Phytochemistry, 49(1), 79−87.   DOI   ScienceOn