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http://dx.doi.org/10.5850/JKSCT.2009.33.6.980

Separation of Chromophoric Substance from Amur Cork Tree Using GC-MS  

Ahn, Cheun-Soon (Dept. of Fashion Industry, University of Incheon)
Publication Information
Journal of the Korean Society of Clothing and Textiles / v.33, no.6, 2009 , pp. 980-989 More about this Journal
Abstract
Amur cork tree was extracted in methanol with the purpose of investigating the most effective extraction procedure for detecting the chromophore using the GC-MS analysis. Different procedures of waterbath and hotplate extractions were carried out and five different GC-MS instrument parameters including the operating temperatures in the GC capillary column and the MSD scan range were tested for their efficiencies. Berberine was determined by the detection of dihydroberberine at 15.0 min r.t. Hotplate was a better device for extracting amur cork tree than waterbath shaker either with or without presoaking in the room temperature. Water was not an adequate extraction medium for the berberine detection. The most effective GC-MS parameter was Method 4; the initial temperature at $50^{\circ}C$ followed by the temperature increase of $23^{\circ}C$/min until $210^{\circ}C$, then increase of $30^{\circ}C$/min until the final temperature reach at $305^{\circ}C$, then hold for 14 minutes to maintain the total run time 24.12 minutes. The MSD scan range for Method 4 was $35\sim400$m/z.
Keywords
Amur cork tree; Berberine; Extraction; GC-MS; Exhumed textiles;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Ahn, C., & Obendorf, S. K. (2006). GC-MS analysis of dyes extracted from turmeric. Fibers and Polymers, 7(2), 158−163   DOI   ScienceOn
2 Giles, C. H. (1965). The fading of colouring matters. Journal of Applied Chemistry, 15, 541−550   DOI
3 So, H. O. (1996). The study of phellodendron amurense rupr. dyeing. The Korean Society of Costume, 28, 121−132
4 Turner, N., Li, J. Y., Gosby, A., To, S. W. C., Cheng, Z., Miyoshi, H., Taketo, M. M., Cooney, G. J., Kraegen, E. W., James, D. E., Hu, L. H., Li, J., & Ye, J. M. (2008a). Berberine and its more biologically available derivative, dihydroberberine, inhibit mitochondrial respiratory complex I. Diabetes, 57(5), 1414−1418   DOI   ScienceOn
5 Orska-Gawry, J., Surowiec, I., Kehl, J., Rejniak, H., Urbaniak- Walczak, K., & Trojanowicz, M. (2003). Identification of natural dyes in archaeological Coptic textiles by liquid chromatography with diode array detection. Journal of Chromatography A, 989, 239−248   DOI   PUBMED   ScienceOn
6 Turner, N., Li, J. Y., Gosby, A., To, S. W. C., Cheng, Z., Miyoshi, H., Taketo, M. M., Cooney, G. J., Kraegen, E. W., James, D. E., Hu, L. H., Li, J., & Ye, J. M. (2008b). Online Appendix. Supplementary methods. Diabetes. Retrieved May 20, 2009, from http://diabetes.diabetesjournals.org/content/vol0/issue2008/images/data/db07-1552/DC1/diabetes.doc
7 Ong, E. S., & Len, S. M. (2003). Pressurized hot water extraction of berberine, baicalein and glycyrrhizin in medicinal plants. Analytica Chimica Acta, 482, 81−89   DOI   ScienceOn
8 Tokusoglu, O., Unal, M. K., & Yildirim, Z. (2003). HPLCUV and GC-MS characterization of the flavonol aglycons quercetin, kaempferol, and myricetin in tomato pastes and other tomato-based products. Acta Chromatographica, 13, 196−207
9 Choi, I. J. (2005). GC/MS를 이용한 현장의 유기미지시료 분석. OSHRI, Korea Occupational Safety and Health Agency. Retrieved May 10, 2009, from http://oshri.kosha.or.kr/
10 Fabbri, D., Chiavari, G., & Ling H. (2000). Analysis of anthraquinoid and indigoid dyes used in ancient artistic works by thermally assisted hydrolysis and methylation in the presence of tetramethylammonium hydroxide. Journal of Anal. Appl. Pyrolysis, 56, 167−178   DOI   ScienceOn
11 Budavari, S., O'Neil, M. J., Smith, A., Heckelman, P. E., & Kinneary, J. F. (1996). The merck index. Whitehouse Station, N.J: Merck & Co, Inc
12 Ahn, C., & Obendorf, S. K. (2004). Dyes on archaeological textiles: Analyzing alizarin and its degradation products. Textile Research Journal, 74(11), 949−954
13 Candan, F., Unlu, M., Tepe, B., Daferera, D., Polissiou, M., Sokmen, A., & Akpulat, H. A. (2003). Antioxidant and antimicrobial activity of the essential oil and methanol extracts of Achillea millefolium subsp. millefolium Afan. (Asteraceae). Journal of Ethnopharmacology, 87, 215−220   DOI   PUBMED   ScienceOn
14 Nam, S. W. (1999). Natural dyeing class. Laboratory of Natural dyeing. Suwan: Sungkeunkwan University
15 Schweppe, H. (1989). Identification of red madder and insect dyes by thin-layer chromatography. In S. H. Zeronian & H. L. Needles (Eds.), Historic textile and paper materials II: Conservation and characterization (pp. 188-219). Washington, DC: American Chemical Society
16 Song, J. F., He, Y. Y., & Guo, W. (2002). Polarographic deteremination of berberine in the presence of $H_2O_2$ in medicinal plants. Journal of Pharmaceutical and Biomedical Analysis, 28, 355−363   DOI   ScienceOn
17 Walton, P., & Taylor, G. (1991). The characterisation of dyes in textiles from archaeological excavations. Chromatography and Analysis, June, 5−7
18 Ong, E. S., Woo, S. O., & Yong, Y. L. (2000). Pressurized liquid extraction of beberine and aristolochic acids in medicinal plants. Journal of Chromatography A, 313, 57−64
19 Ahn, C., & Obendorf, S. K. (2003). Separation of chromophoric chromophoric substance from madder plant under different extraction and analytical conditions. Journal of the Korean Society of Clothing & Textiles, 27(11), 1350-1357