Browse > Article
http://dx.doi.org/10.14478/ace.2022.1023

Physicochemical Properties and Antioxidant Effects of Fucoidans Degraded by Hydrogen Peroxide under Electron Beam at Various Irradiation Doses  

Jeong, Gyeong-Won (Department of Bioenvironmental & Chemical Engineering, Chosun College of Science and Technology)
Choi, Yoo-Sung (Department of Bioenvironmental & Chemical Engineering, Chosun College of Science and Technology)
Publication Information
Applied Chemistry for Engineering / v.33, no.3, 2022 , pp. 322-327 More about this Journal
Abstract
Fucoidans were degraded by hydrogen peroxide under the electron beam (2.5 MeV) with various radiation doses (5 kGy, 10 kGy, 15 kGy, and 20 kGy) at room temperature. The degradation property was analyzed with a gel permeation chromatography (GPC-MALLS) method. An average molecular weight of fucoidan decreased from 99,956 at the irradiation dose of 0 kGy to 6,725 at the irradiation dose of 20 kGy. The solution viscosity of fucoidans showed a similar pattern to the molecular weight change. The number of chain breaks per molecule (N) increased with increasing the irradiation dose and concentration of hydrogen peroxide. The radiation yield of scission value markedly increased with increasing the irradiation dose up to 15 kGy. Also a 10% hydrogen peroxide concentration was more efficient than that of 5%. The structures of degraded fucoidan samples were studied with Fourier transform infrared spectroscopy (FT-IR). The results showed that the degradation process did not significantly change the chemical structure or the content of sulfate group. The sulfur content of each sample was determined with an Elemental Analyzer. With increasing the concentration of hydrogen peroxide, the ratios of sulfur/carbon, hydrogen/carbon, and nitrogen/carbon slightly decreased. The antioxidant activities of fucoidans were investigated based on hydroxyl radical scavenging activities. The ability of fucoidan to inhibit the hydroxyl radical scavenging activity was depended on its molecular weight.
Keywords
Fucoidan; Electron beam; Characterization; Hydroxyl radical; Antioxidant effect;
Citations & Related Records
연도 인용수 순위
  • Reference
1 H. Zegota, The effect of c-irradiation on citrus pectin in N2O and N2O/O2 saturated aqueous solutions, Food Hydrocoll., 13, 51-58 (1999).   DOI
2 H. Zhao, Z. Wang, C. Cheng, L. Yao, L. Wang, W. Lu, and F. Ma, In-vitro free radical scavenging activities of anthocyanins from three berries. J. Med. Plant Res., 5, 7036-7042 (2011).
3 L. Relleve, N. Nagasawa, L. Q. Luan, T. Yagi, C. Aranilla, L. Abad, T. Kume, F. Yoshii, and A. dela Rosaa, Degradation of carrageenan by radiation, Polym. Degrad. Stab., 87, 403-410 (2005).   DOI
4 G. Qu, X. Liu, D. Wang, Y. Yuan, and L. Han, Isolation and characterization of fucoidans from five brown algae and evaluation of their antioxidant activity, J. Ocean Univ. China, 13, 851-856 (2014).   DOI
5 I. Janik, E. Kasprzak, A. Al-Zier, and J. M. Rosiak, radiation crosslinking and scission parameters for poly(vinyl methyl ether) in aqueous solution, Nucl. Instrum. Methods Phys. Res. B, 208, 374-379 (2003).   DOI
6 C. Boisson-Vidal, F. Zemani, G. Calliguiri, I. Galy-Fauroux, S. Colliec-Jouault, D. Helley, and A. M. Fischer, Neoangiogenesis Induced by Progenitor Endothelial Cells: Effect of Fucoidan from Marine Algae, Cardiovasc. Hematol. Agents Med. Chem., 5, 67-77 (2007).   DOI
7 J. M. Wasikiewicz, F. Yoshii, N. Nagasawa, R. A. Wach, and H. Mitomo, Degradation of chitosan and sodium alginate by gamma radiation, sonochemical and ultraviolet method, Radiat. Phys. Chem., 73, 287-295 (2005).   DOI
8 H. S. A. Koh, J. Lu, and W. Zhou, Structure characterization and antioxidant activity of fucoidan isolated from Undaria pinnatifida grown in New Zealand, Carbohydr. Polym., 212, 178-185 (2019).   DOI
9 G. Qu, X. Liu, D. Wang, Y. Yuan, and L. Han, Isolation and characterization of fucoidans from five brown algae and evaluation of their antioxidant activity, J. Ocean Univ. China., 13, 851-856 (2014).   DOI
10 W. S. Choi, K. J. Ahn, D. W. Lee, M. W. Byun, and H. J. Park, Preparation of chitosan oligomers by irradiation, Polym. Degrad. Stab., 78, 533-538 (2002).   DOI
11 O. I. Aruoma, Free radicals, oxidative stress, and antioxidants in human health and disease, J. Am. Oil Chem. Soc., 75, 199-212 (1998).   DOI
12 L. Li, C. Xue, Y. Xue, Z. Li, and X. Fu, The effects of fucoidans from Laminariajaponica on AAPH mediated oxidation of human low- density lipoprotein, Acta Oceanol. Sin., 25, 124-130 (2006).
13 Z. J. Witczak, Polysaccharides in Medicinal Applications, ed. S. Dumitriu, 245-247, Marcel Dekker, Inc., NY, USA (1996).
14 B. Li, F. Lu, X. Wei, and R. Zhao, Fucoidan: Structure and bioactivity, Molecules, 13, 1671-1695 (2008).   DOI
15 M. Lahaye and B. Kaeffer, Seaweed dietary fibres: structure, physicochemical and biological properties relevant to intestinal physiology, Sci. Aliments, 17, 563-584 (1997).
16 P. Ruperez, O. Ahrazem, and J. A. Leal, Potential Antioxidant Capacity of Sulfated Polysaccharides from the Edible Marine Brown Seaweed Fucus vesiculosus, J. Agric. Food Chem., 50, 840-845 (2002).   DOI
17 B. Mulloy, A. C. Ribeiro, A. P. Alves, R. P. Vieira, P. A. S. Mourao, Sulfated fucans from echinoderms have a regular tetrasaccharide repeating unit defined by specific patterns of sulfation at the 0-2 and 0-4 positions, J. Biol. Chem., 269, 22113-22123 (1994).   DOI
18 B. Li, R. X. Zhao, and X. J. Wei, Anticoagulant activity of fucoidan from Hizikia fusiforme, Agro Food. Ind. Hi Tech., 19, 22-24 (2008).
19 C. Yang, D. Chung, I. S. Shin, H. Lee, J. Kim, Y. Lee, and S. You, Effects of molecular weight and hydrolysis conditions on anticancer activity of fucoidans from sporophyll of Undaria pinnatifida, Int. J. Biol. Macromol., 43, 433-437 (2008).   DOI
20 H. Suzuki, K. Suzuki, A. Inoue, and T. A. Ojima, novel oligoalginate lyase from abalone, Haliotis discus hannai, that releases disaccharide from alginate polymer in an exolytic manner, Carbohydr. Res., 341, 1809-1819 (2006).   DOI
21 C. W. Shin and S. G. Choe, Depolymerization of Sodium Alginates by e-Beam Irradiation. Appl. Chem. Eng., 25, 227-232 (2014).   DOI
22 J. Burana-osot, S. Hosoyama, Y. Nagamoto, S. Suzuki, R. J. Linhardt, and T. Toida, Photolytic depolymerization of alginate, Carbohydr. Res., 344, 2023-2027 (2009).   DOI
23 B. Kang, Y. D. Dai, H. Q. Zhang, and D. Chen, Synergetic degradation of chitosan with gamma radiation and hydrogen peroxide, Polym. Degrad. Stab., 92, 359-362 (2007).   DOI