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http://dx.doi.org/10.14478/ace.2021.1084

Effect of Saussurea Lappa Root Extract on Proliferation and Hair Growth-related Signal Pathway in Human Hair Follicle Dermal Papilla Cells  

Chio, Hyoung-Chul (Department of Engineering Chemistry, Chungbuk National University)
Jeong, Noh-Hee (Department of Engineering Chemistry, Chungbuk National University)
Publication Information
Applied Chemistry for Engineering / v.32, no.6, 2021 , pp. 647-652 More about this Journal
Abstract
In this study, Saussurea Lappa roots were extracted using ethanol and n-hexane, and also the effects on proliferation of human hair dermal papilla cells and fibroblast and related signaling pathway were evaluated. 3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide (MTT) assay was conducted for cell proliferation effect of Saussurea Lappa root extract, and extracellular signal-related kinase (ERK), serine/threonine protein kinase (Akt), wingless-related integration site (Wnt)/𝛽-catenin signaling pathway, and 5𝛼-reductase expression through western blot analysis were measured. Saussurea Lappa root extract significantly increased human hair dermal papilla cells and propagation of fibroblast, promoted phosphorylation of ERK and Akt that get involved in cell proliferation. Additionally, Saussurea Lappa root extract significantly decreased promotion of Akt phosphorylation and cell proliferation by MEK/ERK inhibitor PD98059 and PI3K/Akt inhibitor LY294002. Also, Saussurea Lappa root extract induced intranuclear 𝛽-catenin accumulation by promoting phosphorylation of 𝛽-catenin (Ser552, 675) through phosphorylation of GSK-3𝛽 (Ser9), and suppressed activation of 5𝛼-reductase type I and II. Overall, Saussurea Lappa root induces cell proliferation through vitalization of ERK and Akt route of human hair dermal papilla cells and fibroblast and apoptosis defense mechanism, and can be helpful in hair loss prevention and hair growth by vitalizing the 𝛽-catenin signaling pathway and inhibiting activation of 5𝛼-reductase, which can be used as a potential hair care products.
Keywords
Saussurea Lappa root extract; Human hair dermal papilla cells; Cell proliferation; Signaling pathway; Hair care;
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1 H. Rastegar, H. Ahmadi Ashtiani, M. Aghaei, A. Ehsani, and B. Barikbin, Combination of herbal extracts and platelet-rich plasma induced dermal papilla cell proliferation: involvement of ERK and A kt pathways, J. Cosmet. Dermatol., 12, 116-122 (2013).   DOI
2 S. H. Lee and J. R. Lee, Association of diffuse hair loss of adult male on stress, self-confidence, and depression, J. Korean Soc. Cosmetol., 16, 1171-1179 (2010).
3 M. H. Kwack, J. S. Ahn, M. K. Kim, J. C. Kim, and Y. K. Sung, Preventable effect of L-threonate, an ascorbate metabolite, on androgen-driven balding via repression of dihydrotestosteroneinduced dickkopf-1 expression in human hair dermal papilla cells, BMB reports, 43, 688-692 (2010).   DOI
4 V. A. Botchkarev and J. Kishimoto, Molecular control of epithelial-mesenchymal interactions during hair follicle cycling, J. Invest. Dermatol., 8, 46-55 (2003).   DOI
5 M. B. Morgan, and P. Rose, An investigation of apoptosis in androgenetic alopecia, Ann. Clin. Lab. Sci., 33, 107-112 (2003).
6 K. J. McClennan and A. Markham, Finasteride: A review of its use in male pattern baldness, Drugs, 57, 111-126 (1999).   DOI
7 H. Wosicka, and K. Cal, Targeting to the hair follicles: current status and potential, J. Dermatol. Sci., 57, 83-89 (2010).   DOI
8 J. I. Yoon, S. M. Al-Reza, and S. C. Kang, Hair growth promoting effect of Zizyphus jujuba essential oil, Food Chem. Toxicol., 48, 1350-1354 (2010).   DOI
9 N. Dufton, and M. Perretti, Therapeutic anti-inflammatory potential of formyl-peptide receptor agonists, Pharmacol. Ther., 127, 175-188 (2010).   DOI
10 A. Ali, and J. M. Martin, Hair growth in patients alopecia areata totalis after treatment with simvastatin and ezetimibe, J. Drugs Dermatol., 9, 62-64 (2010).
11 S. Inui, Y. Fukuzato, T. Nakajima, K. Yoshikawa, and S. Itami, Androgen-inducible TGFβ1 from balding dermal papilla cells inhibits epithelial cell growth: a clue to understanding paradoxical effects of androgen on human hair growth, FASEB J., 16, 1967-1969 (2002).   DOI
12 J. Kishimoto, R. E. Burgeson, and B. A. Morgan, Wnt signaling maintains the hair-inducing activity of the dermal papilla, Genes Dev., 14, 1181-1185 (2000).   DOI
13 Y. J. Jeon, H. S. Lee, J. H. Ko, K. M. An, S. W. Yu, J. H. Kang, B. Y. Hwang, and T. Y. Kim, Inhibitory effects of dehydrocostuslactone isolated from Saussureae radix on CDK2 activity, Korean J. Pharmacogn., 36, 97-101 (2005).
14 N. Le Floch, C. Rivat, O. De Wever, E. Bruyneel, M. Mareel, T. Dale, and C. Gespach, The proinvasive activity of Wnt-2 is mediated through a noncanonical Wnt pathway coupled to GSK-3β and c-Jun/AP-1 signaling, FASEB J., 19, 144-146 (2005).   DOI
15 Y. M. Park, J. S. Han, Y. M. Park, and J. S. Han, A study on the utilization of Dendropanax morbifera Lev. leaf extract for material of functional cosmetics and hair growth products, Asian J. Beauty Cosmetol., 14, 277-288 (2016).   DOI
16 S. Mitsui, A. Ohuchi, M. Hotta, R. Tsuboi, and H. Ogawa, Genes for a range of growth factors and cyclin-dependent kinase inhibitors are expressed by isolated human hair follicles, Brit. J. Dermatol., 137, 693-698 (1997).   DOI
17 T. Andl, S. T. Reddy, T. Gaddapara, and S. E. Millar, WNT signals are required for the initiation of hair follicle development, Dev. Cell, 2, 643-653 (2002).   DOI
18 H. S. Hwang, T. H. Hwang, A. J. Pyo, and E. H. Ju, Anti-oxidant efficacy and effects on expression of growth factors in human hair follicle dermal papilla cells of Rosa multiflora root extracts, Asian J. Beauty Cosmetol., 15, 146-158 (2017).   DOI
19 M. S. Lee, D. G. Ryu, and K. B. Kwon, Anti-inflammatory effects of Saussurea Lappa extracts in murine macrophages, J. Physiol. Pathol. Korean Med., 25, 275-279 (2011).
20 T. R. Kwon, C. T. Oh, E. J. Choi, H. M. Park, H. J. Han, H. J. Ji, and B. J. Kim, Human placental extract exerts hair growth-promoting effects through the GSK-3β signaling pathway in human dermal papilla cells. Int. J. Mol. Med., 36, 1088-1096 (2015).   DOI
21 S. J. Jeong, T. Itokawa, M. Shibuya, M. Kuwano, M. Ono, R. Higuchi, and T. Miyamoto, Costunolide, a sesquiterpene lactone from Saussurea lappa, inhibits the VEGFR KDR/Flk-1 signaling pathway, Cancer Lett., 187, 129-133 (2002).   DOI
22 P. D. Ray, B. W. Huang, and Y. Tsuji, Reactive oxygen species(ROS) homeostasis and redox regulation in cellular signaling. Cell. Signal., 24, 981-990 (2012).   DOI
23 J. I. Kang, J. S. Moon, E. J. Kim, Y. S. Koh, E. S. Yoo, and H. K. Kang, The Hair Growth Effects of Wheat Bran. Korean J. Pharmacogn., 44, 384-390 (2013).
24 H. Rastegar, H. A. Ashtiani, M. Aghaei, B. Barikbin, and A. Ehsani, Herbal extracts induce dermal papilla cell proliferation of human hair follicles. Ann. Dermatol., 27, 667-675 (2015).   DOI
25 T. Hagemann, B. Schlutter-Bohmer, J. P. Allam, T. Bieber, and N. Novak, Positive lymphocyte transformation test in a patient with allergic contact dermatitis of the scalp after short-term use of topical minoxidil solution, Contact Derm., 53, 53-55 (2005).   DOI
26 D. B. Kim, E. Y Ahn, and E. J. Kim, Improvement of insulin resistance by curcumin in high fat diet fed mice, JCCT, 4, 315-323 (2018).
27 K. S. Stenn, The molecular and structural biology of hair: introduction, Ann. N. Y. Acad. Sci.,, 26, 11-13 (1991).