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http://dx.doi.org/10.14478/ace.2021.1058

Preparation and Characterization of Chitosan-coated PLGA Nanoparticle  

Yu, Su-Gyeong (Department of Polymer Science and Engineering, Sunchon National University)
Nah, Jae-Woon (Department of Polymer Science and Engineering, Sunchon National University)
Jeong, Gyeong-Won (Department of Bioenvironmental & Chemical Engineering, Chosun College of Science and Technology)
Publication Information
Applied Chemistry for Engineering / v.32, no.5, 2021 , pp. 509-515 More about this Journal
Abstract
In this study, poly lactic-co-glycolic acid (PLGA) nanoparticles (PNP) were prepared through double (w/o/w) emlusion and emulsifying solvent-evaporation technique using PLGA, which has biocompatibility and biodegradability. To maximize stability and bioavailability of the particles, chitosan-coated PLGA nanoparticles (CPNP) were prepared by charge interaction between PNP and chitosan. We demonstrated that CPNP can be utilized as a drug carrier of oral administration. The chemical structure of CPNP was analyzed by 1H-NMR and FT-IR, and all characteristic peaks appeared, confirming that it was successfully prepared. In addition, particle size and zeta potential of CPNP were analyzed using dynamic light scattering (DLS) while morphological images were obtained using transmission electron microscope (TEM). Thermal decomposition behavior of CPNP was observed through thermogravimetric analysis (TGA). In addition, the cytotoxicity of CPNP was confirmed by MTT assay at HEK293 and L929 cell lines, and it was proved that there is no toxicity confirmed by the cell viability of above 70% at all concentrations. These results suggest that the CPNP developed in this study may be used as an oral drug delivery carrier.
Keywords
Chitosan; PLGA; Oral delivery carrier; Stability; Bioavailability;
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