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http://dx.doi.org/10.14478/ace.2020.1095

Characterization of pH Dependent Properties of mCherry Mutant, I202T  

Lee, Sangmin (Department of Chemical Engineering, Hongik University)
Chung, Minsub (Department of Chemical Engineering, Hongik University)
Publication Information
Applied Chemistry for Engineering / v.32, no.1, 2021 , pp. 10-14 More about this Journal
Abstract
mCherry is one of the well-understood red fluorescent proteins which has a similar tertiary structure as GFPs, but pH resistant due to the lack of hydrogen bond network. Whereas mCherry-I202T showed far-red fluorescence and also pH sensitive property because of the additional hydrogen bond formed by substituting Ile of 202 amino acid sequence on mCherry with Thr. In order to verify the pH sensitive characteristic of mCherry-I202T owing to the extension of hydrogen bond, UV-vis spectrum was measured over the range of acidic to basic pH. We also demonstrate further possibilities of applying mCherry-I202T as a pH sensor.
Keywords
Far-red fluorescent proteins; mCherry; Mutagenesis; H-bond network; pH sensor;
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