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http://dx.doi.org/10.14478/ace.2019.1029

Thiolated Protein A-functionalized Bimetallic Surface Plasmon Resonance Chip for Enhanced Determination of Amyloid Beta 42  

Kim, Hyung Jin (Department of Biomedical Engineering, Daegu Catholic University)
Kim, Chang-Duk (Department of Physics, Kyungpook National University)
Sohn, Young-Soo (Department of Biomedical Engineering, Daegu Catholic University)
Publication Information
Applied Chemistry for Engineering / v.30, no.3, 2019 , pp. 379-383 More about this Journal
Abstract
The capability of detecting amyloid beta 42 ($A{\beta}42$), a biomarker of Alzheimer's disease, using a thiolated protein A-functionalized bimetallic surface plasmon resonance (SPR) chip was investigated. An optimized configuration of a bimetallic chip containing gold and silver was obtained through calculations in the intensity measurement mode. The surface of the SPR bimetallic chip was functionalized with thiolated protein A for the immobilization of $A{\beta}42$ antibody. The response of the thiolated protein A-functionalized bimetallic chip to $A{\beta}42$ in the concentration range of 50 to 1,000 pg/mL was linear. Compared to protein A without thiolation, the thiolated protein A resulted in greater sensitivity. Therefore, the thiolated protein A-functionalized bimetallic SPR chip can be used to detect very low concentrations of the biomarker for Alzheimer's disease.
Keywords
Surface plasmon resonance; Amyloid beta 42; Alzheimer's disease; Intensity measurement;
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