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http://dx.doi.org/10.14478/ace.2018.1077

Antimicrobial and Antioxidant Activities of Perilla frutescens var. acuta Extract and Its Fraction and Their Component Analyses  

Jeong, Hyo Jin (Department of Fine Chemistry, Cosmetic R&D center, Seoul National University of Science and Technology)
Xuan, Song Hua (Department of Fine Chemistry, Cosmetic R&D center, Seoul National University of Science and Technology)
Song, Ba Reum (Department of Fine Chemistry, Cosmetic R&D center, Seoul National University of Science and Technology)
Lee, Sang Lae (Department of Fine Chemistry, Cosmetic R&D center, Seoul National University of Science and Technology)
Lee, Yun Ju (Department of Fine Chemistry, Cosmetic R&D center, Seoul National University of Science and Technology)
Park, Soo Nam (Department of Fine Chemistry, Cosmetic R&D center, Seoul National University of Science and Technology)
Publication Information
Applied Chemistry for Engineering / v.29, no.6, 2018 , pp. 716-725 More about this Journal
Abstract
In this study, antimicrobial and antioxidative activities of Perilla frutescens var. acuta were investigated with 50% ethanol and the ethyl acetate fraction and also the components were analyzed. The minimum inhibitory concentration (MIC) of the ethyl acetate fraction for both Staphylococcus aureus and Pseudomonas aeruginosa were $78{\mu}g/mL$, indicating high antimicrobial effects. The free radical scavenging activity ($FSC_{50}$) and the reactive oxygen species (ROS) scavenging activity ($OSC_{50}$) in $Fe^{3+}-EDTA/H_2O_2$ system values of the ethyl acetate fraction were $25.90{\mu}g/mL$ and $1.40{\mu}g/mL$, respectively. After the cell damage induced by $400mJ/cm^2$ UVB irradiation, the cytoprotective effect of the ethyl acetate fraction of P. frutescens var. acuta showed the concentration dependent manner ranging from 2.0 to $16.0{\mu}g/mL$. The intracellular ROS inhibitory activity in HaCaT cells decreased to 28.6% and 40.7% for the 50% ethanol extract and ethyl acetate fraction, respectively at the concentration of $32{\mu}g/mL$. Components of rosmarinic acid, luteolin, apigenin, caffeic acid and ethyl caffeate were identified in the ethyl acetate fraction. These results suggest that the extract and fraction of P. frutescens var. acuta may be applied to the field of cosmetics as a natural material that protects the skin from an external environment by having antimicrobial and antioxidative activities.
Keywords
Perilla frutescens; antioxidative activity; reactive oxygen species; antimicrobial effect; flavonoid;
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Times Cited By KSCI : 4  (Citation Analysis)
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1 E. A. Grice and J. A. Segre, The skin microbiome, Nat. Rev. Microbiol., 9, 244-253 (2011).   DOI
2 M. R. Yi, A. L. Jeon, C. H. Kang, and H. J. Bu, Antioxidant, antimicrobial and anti-inflammatory activities of essential oil from Erigeron annuus L. flower, J. Korean Oil. Chem. Soc., 33, 717-725 (2016).   DOI
3 J. H. Kim, M. J. Kim, S. K. Choi, S. H. Bae, S. K. An, and Y. M. Yoon, Antioxidant and antimicrobial effects of lemon and eucalyptus essential oils against skin floras, J. Soc. Cosmet. Sci. Korea, 37, 303-308 (2011).
4 D. H. Kim, Y. C. Kim, and U. K. Choi, Optimization of antibacterial activity of Perilla frutescens var. acuta leaf against Staphylococcus aureus using evolutionary operation factorial design technique, Int. J. Mol. Sci., 12, 2395-2407 (2011).   DOI
5 S. H. Park, J. S. Seong, K. S. Lee, Y. M. Park, S. H. Xuan, M. Y. Cha, H. C. Kang, and S. N. Park, Antioxidant and cellular protective effects of Parthenocissus tricuspidata stem extracts fermented by Lactobacillus pentosus, J. Soc. Cosmet. Sci. Korea, 43, 255-263 (2017).
6 M. Kim, Y. G. Park, H. J. Lee, S. J. Lim, and C. W. Nho, Youngiasides A and C isolated from Youngia denticulatum inhibit UVB-induced MMP expression and promote type I procollagen production via repression of MAPK/AP-1/NF-kappaB and activation of AMPK/Nrf2 in HaCaT cells and human dermal fibroblasts, J. Agric. Food Chem., 63, 5428-5438 (2015).   DOI
7 R. C. Alves, A. S. G. Costa, M. Jerez, S. Casal, J. Sineiro, M. J. Nunez, and B. Oliveira, Antiradical activity, phenolic profile, and hydroxymethylfurfural in espresso coffee: Influence of technological factors, J. Agric. Food. Chem., 58, 12221-12229 (2010).   DOI
8 A. Rebaya, S. I. Belghith, B. Baghdikian, V. M. Leddet, F. Mabrouki, E. Olivier, J. K. Cherif, and M. T. Ayadi, Total phenolic, total flavonoid, tannin content, and antioxidant capacity of Halimium halimifolium (Cistaceae), J. Appl. Pharm. Sci., 5, 52-57 (2014).
9 D. Stojiljkovic, D. Pablobic, and I. Arsic, Oxidative stress, skin aging and antioxidant therapy, Acta Fac. Med. Naissensis, 31, 207-217 (2014).   DOI
10 A. Amaro-Ortiz, B. Yan, and J. A. D'Orazio, Ultraviolet radiation, aging and the skin: prevention of damage by topical cAMP manipulation, Molecules, 19, 6202-6219 (2015).
11 H. A. Yu and C. D. Kim, Applicability of Lindera obtusiloba flower extracts as cosmetic ingredients, Asian. J. Beauty Cosmetol., 15, 132-144 (2017).   DOI
12 A. Kammeyer and R. M. Luiten, Oxidation events and skin aging, Ageing Res. Rev., 21, 16-29 (2015).   DOI
13 E. Y. Choi, Effect of phenoxyethanol and alkane diol mixture on the antimicrobial activity and antiseptic ability in cosmetics, Korean J. Aesthet. Cosmetol., 13, 213-220 (2015).
14 Y. H. Yeo and C. H. Park, Cosmetics preservation and moisturizing effect by methanol extracts of Scutellaria baicalensis George and Liriope platyphylla, Korean Soc. Biotechnol. Bioeng. J., 29, 372-379 (2014).
15 S. Pillai, C. Oresajo, and J. Hayward, Ultraviolet radiation and skin aging: roles of reactive oxygen species, inflammation and protease activation, and strategies for prevention of inflammation-induced matrix degradation - a review, Int. J. Cosmet. Sci., 27, 17-34 (2005).   DOI
16 C. Namngam and P. Pinsirodom, Antioxidant properties, selected enzyme inhibition capacities, and a cosmetic cream formulation of Thai mango seed kernel extracts, Trop. J. Pharm. Res., 16, 9-16 (2017).   DOI
17 M. Wlaschek, I. Tantcheva-Poor, L. Naderi, W. J. Ma, A. Schneider, Z. Razi-Wolf, J. Schuller, and K. Scharffetter- Kochanek, Solar UV irradiation and dermal photoaging, J. Photochem. Photobiol., 63, 41-51 (2001).   DOI
18 W. Y. Song and J. H. Choi, Total phenols, flavonoid contents, and antioxidant activity of Spirodela polyrhixa extracts, J. Life Sci., 27, 180-186 (2017).   DOI
19 M. L. Soto, M. Parada, E. Falque, and H. Dominguez, Personal-care products formulated with natural antioxidant extracts, Cosmetics, 5, 13-22 (2018).   DOI
20 E. C. Kim, S. Y. Ahn, E. S. Hong, G. H. Li, E. K. Kim, and K. H. Row, Extraction of whitening agents from natural plants and whitening effect, J. Korean Ind. Eng. Chem., 16, 348-353 (2005).
21 Y. M. Chiang, C. P. Lo, Y. P. Chen, S. Y. Wang, N. S. Yang, Y. H. Kuo, and L. F. Shyur, Ethyl caffeate suppresses NF-${\kappa}$B activation and its downstream inflammatory mediators, iNOS, COX-2, and $PGE_2$ in vitro of in mouse skin, Br. J. Pharmacol., 146, 352-363 (2005).   DOI
22 M. J. Ha and S. H. You, Bioactive characteristics of extracts of Opuntia humifusa fruit as functional cosmetic ingredients, Asian. J. Beauty Cosmetol., 14, 463-472 (2016).   DOI
23 L. Meng, Y. F. Lozano, E. M. Gaydou, and B. Li, Antioxidant activities of polyphenols extracted from Perilla frutescens varieties, Molecules, 14, 133-140 (2009).
24 N. S. Kang and J. H. Lee, Characterisation of phenolic phytochemicals and quality changes related to the harvest times from the leaves of Korean purple perilla (Perilla frutescens), Food Chem., 124, 556-562 (2011).   DOI
25 D. Y. Park and K. Y. Lee, Evaluation of the cosmeceutical activity of ethanol extracts from Perilla frutescens var. acuta, J. Korea Acad. Ind. Coop. Soc., 18, 513-517 (2017).
26 C. A. Rice-evans, N. J. Miller, P. G. Bolwell, P. M. Bramley, and J. B. Pridha, The relative antioxidant activities of plant-derived polyphernolic flavonoids, Free Rad. Res., 22, 375-383 (1995).   DOI
27 K. Griffiths, B. B. Aggarwal, R. B. Singh, H. S. Buttar, D. Wilson, and F. D. Meester, Food antioxidants and their anti-inflammatory properties: a potential role in cardiovascular diseases and cancer prevention, Diseases, 4, 28-42 (2016).   DOI
28 M. H. Kim, W. W. Kang, N. H. Lee, D. J. Kwoen, and U. K. Choi, Antioxidant activities of extract with water and ethanol of Perilla frutescens var. acuta kudo leaf, J. Korean Soc. Appl. Biol. Chem., 50, 327-333 (2007).
29 J. S. Bae, M. R. Han, H. S. Shin, M. K. Kim, C. Y. Shin, D. H. Lee, and J. H. Chung, Perilla frutescens leaves extract ameliorates ultraviolet radiation-induced extracellular matrix damage in human dermal fibroblasts and hairless mice skin, J. Ethnopharmacol., 195, 334-342 (2017).   DOI
30 J. S. You, S. Y. Kim, S. H. Kim, and T. Y. Shin. Antiallergic and anti-inflammatory effects of Perilla frutescens var. acuta. Korean J. Pharmacogn., 43, 163-166 (2012).
31 M. H. Kim, N. H. Lee, M. H. Lee, D. J. Kwon, and U. K. Choi, Antimicrobial activity of aqueous ethanol extracts of Perilla frutescens var. acuta leaf, Korean J. Food. Cult., 22, 266-273 (2007).
32 Y. Nakamura, Y. Ohto, A. Murakami, and H. Ohigashi, Superoxide scavenging activity of rosmarinic acid from Perilla frutescens britton var. acuta f. viridis, J. Agric. Food. Chem., 46, 4545-4550 (1998).   DOI