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http://dx.doi.org/10.14478/ace.2017.1097

Cellular Protective Effects and Mechanisms of Kaempferol and Nicotiflorin Isolated from Annona muricata against 1O2-induced Damage  

Park, So Hyun (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology)
Shin, Hyuk Soo (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology)
Lee, Nan Hee (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology)
Hong, In Kee (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology)
Park, Soo Nam (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology)
Publication Information
Applied Chemistry for Engineering / v.29, no.1, 2018 , pp. 49-55 More about this Journal
Abstract
In this study, we investigated the cellular protective effects and mechanisms of nicotiflorin and its aglycone kaempferol isolated from Annona muricata. The protective effect of these components against $^1O_2$-induced cell damage was also studied by using L-ascorbic acid and (+)-${\alpha}$-tocopherol as controls. Kaempferol exhibited the most potent protective effect, followed by (+)-${\alpha}$-tocopherol and nicotiflorin. L-Ascorbic acid did not exhibit any cellular protective effects. To elucidate the mechanism underlying protective effects, the quenching rate constant of the singlet oxygen, free radical-scavenging activity, ROS-scavenging activity, and uptake ratio of the erythrocyte membrane were measured. The results showed that the cell membrane penetration is a key factor determining the cellular protective effect of kaempferol and its glycoside nicotiflorin. The result from L-ascorbic acid demonstrated that the cellular protective effect of a compound depends on its ability to penetrate the cell membrane and is independent of its antioxidant capacity. In addition, it is suggested that cellular protective effects of kaempferol and (+)-${\alpha}$-tocopherol depend not only on the cell permeability, but also on free radical- and ROS-scavenging activities. These results indicate that the cell permeability and free radical- and ROS- scavenging activities of antioxidants are major factors affecting the protection of cell membranes against the oxidative damage induced by photosensitization reaction.
Keywords
cellular protective effect; kaempferol; nicotiflorin; antioxidant mechanism;
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