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http://dx.doi.org/10.14478/ace.2016.1131

Antioxidant and Skin Whitening Effect of Graviola (Annona muricata) Leaf Extracts  

Jo, Eun Hee (Department of Pharmaceutical Engineering, College of Medical and Life Science, Silla University)
Kim, In Hae (Department of Pharmaceutical Engineering, College of Medical and Life Science, Silla University)
Lee, Jae Hwa (Department of Pharmaceutical Engineering, College of Medical and Life Science, Silla University)
Publication Information
Applied Chemistry for Engineering / v.28, no.2, 2017 , pp. 198-205 More about this Journal
Abstract
In this study, graviola leaf extracts (GLE) was investigated for the effect of antioxidant, antibacterial, whitening, anti-wrinkle. The antioxidant effect of GLE was measured by an electron donating ability assay. As a result, GLE increased the electron donating ability in a concentration-dependent manner. The antibacterial effect of GLE was found to show the higher antibacterial effect in Methicillin-resistant Staphylococcus aureus CCARM3115 compared with that of ampicillin by a paper disc method. The whitening effect of GLE was also measured by tyrosinase inhibition assay, and it was found that the tyrosinase activity of GLE decreased as the concentration increased. The inhibition activity of tyrosinase involved in hydroxylation reaction which is related to converting L-tyrosine to (DOPA) was higher than that of arbutin's at the concentration ranging from 125 to $250{\mu}g/mL$. In addition, GLE reduced melanin contents of B16-F10 melanoma cells in a dose-dependant manner and decreased to about 76.7% at a concentration of $100{\mu}g/mL$ Regarding wrinkling formation of GLE, an elastase inhibition assay was performed. As a result, GLE and ursolic acid were 10.5% and 56.5%, respectively under the identical concentration. These results suggest that GLE has significant antioxidant and whitening activities, and also may be potentially used as a therapeutic agent for hyperpigmentation treatment as an ingredient of whitening cosmetics.
Keywords
annona muricata; graviola; whitening; tyrosinase;
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