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http://dx.doi.org/10.14478/ace.2015.1075

Hydrogen Peroxide Sensitive Biosensors Based on Mugwort-Peroxidase Entrapped in Carbon Pastes  

Yoon, Kil Joong (Department of Applied Chemistry, Cheongju University)
Publication Information
Applied Chemistry for Engineering / v.26, no.5, 2015 , pp. 624-629 More about this Journal
Abstract
A biosensor including the homogenized tissue of mugwort embedded in carbon paste, which senses hydrogen peroxide, was constructed and its electrochemical properties were validated using voltammetry. The good linearity of Hanes-Woolf plot implied that the reduction reaction of substrate was catalyzed by mugwort peroxidase at the electrode surface. Also the small value of symmetry factor, 0.28, indicated that electrochemical kinetics of the sensor is very sensitive to the change of electrode potential. Many experimental results collected above proved that the dissociation of hydrogen peroxide is dependent on the catalytic power of mugwort peroxidase qualitatively and quantitatively at the surface of the mugwort electrode. It is our firm belief that the marketed HRP can be replaced with mugwort tissue.
Keywords
mugwort; hydrogen peroxide; peroxidase; biosensor; CSM rubber;
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Times Cited By KSCI : 11  (Citation Analysis)
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