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Phosphate Removal Using Recombinant Bacteria with Cytoplasmic Phosphate Binding Protein  

Choi, Suk Soon (Department of Biological and Environmental Engineering, Semyung University)
Ha, Jeong Hyub (School of Environmental Science and Technology, Pohang University of Science and Technology)
Cha, Hyung Joon (Department of Chemical Engineering, Pohang University of Science and Technology)
Publication Information
Applied Chemistry for Engineering / v.24, no.5, 2013 , pp. 558-561 More about this Journal
Abstract
In the present work, we constructed a recombinant Escherichia coli with cytoplasmic-expressed phosphate-binding protein (PBP) and investigated its phosphate removal in water phase. When the recombinant bacteria were cultured for 6 h to treat phosphate, the removal efficiencies were 90, 49, and 41% for the treatment of 1.0, 1.5, and 2.0 mg/L phosphate, respectively, indicating good specific phosphate removal of our developed system. Also, cell densities of 2.5 and 5.0 Optical density resulted in high phosphate removal efficiencies and ~80% of 2.0 mg/L phosphate was efficiently removed. A novel biotechnology developed in this study could be effectively employed for resolving eutrophication problem in water body.
Keywords
cytoplasmic phosphate-binding protein; biological phosphate removal; removal efficiency of phosphate;
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