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Isolation and Culture Conditions of Hydrogen-producing Bacterium from Sewage Sludge  

Woo, Dae-Sik (Department of Bioscience and Biotechnology, College of Medical and Life Science, Silla University)
Joo, Hyun (Department of Physiology, College of Medicine, Inje University)
Lee, Jae-Hwa (Department of Bioscience and Biotechnology, College of Medical and Life Science, Silla University)
Publication Information
Applied Chemistry for Engineering / v.24, no.4, 2013 , pp. 407-411 More about this Journal
Abstract
Hydrogen producing bacterium, strain AS12 was isolated from the sludge of the anaerobic wastewater treatment process of south sewage treatment plant, Busan city. Phylogenetic analysis based on 16S rRNA sequence studies indicated that AS12 belonged to the genus Escherichia coli sp.. The optimum temperature and pH for hydrogen production were $35^{\circ}C$ and 8.0, respectively. The impact of the types and concentrations of carbon and nitrogen sources in the media on hydrogen production was investigated. The optimum carbon and nitrogen concentrations were 10 g/L of galactose and 5 g/L of peptone, respectively.
Keywords
hydrogen production; Escherichia coli; galactose; biological hydrogen production; facultative anaerobe;
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