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A Comparative Study on the Effect of Commercialized Immobilized Lipases on the Selective Synthesis of 1,3-Diglyceride  

Chung, Dae-Won (Department of Polymer Engineering, College of Engineering, Suwon University)
Cho, Mi-Hye (Department of Polymer Engineering, College of Engineering, Suwon University)
Publication Information
Applied Chemistry for Engineering / v.21, no.4, 2010 , pp. 452-456 More about this Journal
Abstract
It is known that 1,3-diglyceride (1,3-DG) hardly accumulates inside human body because the metabolism of 1,3-DG is entirely different from that of general fats such as triglycerides (TG). This research focuses on the selective synthesis of 1,3-DG by the esterification reaction using an immobilized lipase. For a reaction between glycerin and oleic acid (OA) with a mole ratio of 1 : 2 under vacuum, changes in the compositions of monoglyceride (MG), TG and DG and the contents of 1,3-isomers in DG were investigated, as a function of reaction temperature and the amount of lipase. The reactivities determined by the rate of the consumption of OA became higher with the increase in temperature and the amount of lipase. When the results were compared with those obtained in the earlier study where Novozym was applied as an immobilized lipase, the reactivity was higher for Novozym, on the other hand, selectivity to 1,3-DG was much higher for Lipozyme. Especially, it is remarkable that 1,3-DG content in total DG reached to 98% in the reaction carried out at $50^{\circ}C$ using Lipozyme.
Keywords
1,3-diglyceride; lipase; esterification; oleic acid; selective synthesis;
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