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A Study on the Quantification of PrP 106-126 Peptide by Fluorescamine and Alpha-imager  

Jeong, Keunhong (Department of Chemistry, Korea Military Academy)
Chung, Woo Young (Department of Chemistry, Korea Military Academy)
Kye, Young Sik (Department of Chemistry, Korea Military Academy)
Publication Information
Applied Chemistry for Engineering / v.20, no.6, 2009 , pp. 628-631 More about this Journal
Abstract
Recent social interests on mad cow disease and Creutzfelt-Jacob disease lead researcher' focus onto pathogenic prion proteins causing those diseases. The purpose of this study is to introduce a novel method for micromolecular level quantification of PrP 106-126 peptide, which is a part of prion protein. Fluorescamine has been chosen due to its fluorescence emission characteristics upon reaction with primary amines and Alpha-imager is used to detect the intensity of fluorescence. We succeeded in setting optimal conditions for quantification of PrP 106-206, amyloidogenic prion peptide, at micromolecular level. This study will contribute to identify prion protein aggregation inhibitor and develop new prion protein drug.
Keywords
PrP 106-126; fluorescamine; alpha-imager;
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1 M. Bradford, Anal. Biochem., 72, 248 (1976)   DOI   PUBMED   ScienceOn
2 E. E. Hartree, Anal. Biochem., 48, 422 (1972)   DOI   PUBMED   ScienceOn
3 J. Collinge, J. Neurol. Neurosurg. Psychiatry, 76, 906 (2005)   DOI   ScienceOn
4 S. Mead, Eur. J. Hum. Genet., 14, 273 (2006)   DOI   ScienceOn
5 R. G. Will, J. W. Ironside, M. Zeidler, S. N. Cousens, K. Estibeiro, A. Alperovitch, S. Poser, M. Pocchiari, A. Hofman, and P. G. Smith, Lancet., 347, 921 (1996)   DOI   ScienceOn
6 J. Collinge, K. C. L. Sidle, J. Meads, J. Ironside, and A. F. Hill, Nature, 383, 685 (1996)   DOI   ScienceOn
7 A. F. Hill, M. Desbruslais, S. Joiner, K. C. L. Sidle, I. Gowland, and J. Collinge, Nature, 389, 448 (1997)   DOI   PUBMED   ScienceOn
8 F. Tagliavini, G. Forloni, P. D'Ursi, O. Bugiani, and M. Salmona, Adv. Protein Chem., 57, 171 (2001)   DOI   PUBMED
9 C. E. Jones, S. R. Abdelraheim, D. R. Brown, and J. H. Viles, J. Biol. Chem., 279, 32018 (2004)   DOI   ScienceOn
10 J. Suh, S. H. Yoo, M. G. Kim, K. Jeong, J. Y. Ahn, M. S. Kim, P. S. Chae, T. Y. Lee, J. Lee, J. Lee, Y. A. Jang, and E. H. Ko, Angew. Chem. Int. Ed., 46, 7064 (2007)   DOI   ScienceOn
11 K. Jeong, M. K. Kim, W. Y. Chung, Y. S. Kye, and J. Suh, J. Ind. Eng. Chem., 15, 342 (2009)   DOI   ScienceOn
12 M. E. Bruce, R. G. Will, J. W. Ironside, I. McConnell, D. Drummond, A. Suttie, L. McCardle, A. Chree, J. Hope, C. Birkett, S. Cousens, H. Fraser, and C. J. Bostock, Nature, 389, 498 (1997)   DOI   ScienceOn
13 L. A. Sikkink and M. Ramirez-Alvarado, Biophy. Chem., 13525 (2008)
14 J. C. Blackstock, Biochemistry, Butterworth-Heinemann, Boston (1998)
15 M. W. McCaman and E. Robins, J. Lab. Clin. Med., 59, 885 (1962)
16 P. Deriziotis and S. J. Tabrizi, Biochim Biophys. Acta, 1782, 713 (2008)   DOI   PUBMED   ScienceOn
17 C. M. Stoscheck, Meth. Enz., 182, 50 (1990)   DOI
18 O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall, J. Biol. Chem., 193, 265 (1951)   PUBMED
19 K. Wilson and J. Walker, Practical Biochemistry: Principles and Techniques, Cambridge University Press, Cambridge (2000)
20 J. Suh, J. Lee, S. H. Yoo, K. Jeong, T. Y. Lee, and J. Y. Ahn, Bull. Korean Chem. Soc., 29, 884 (2008)
21 J. Suh, W. S. Chei, T. Lee, M. G. Kim, S. H. Yoo, K. Jeong, and J. Y. Ahn, J. Biol. Inorg. Chem., 13, 693 (2008)   DOI   ScienceOn
22 J. Wadsworth and J. Collinge, Biochim. Biophys. Acta, 1772, 598 (2007)   DOI   PUBMED   ScienceOn
23 S. Udenfriend, S. Stein, P. B$\ddot{o}$hlen, W. Dairman, W. Leimgruber, and M. Weigele, Science, 178, 871 (1971)
24 J. W. Wilesmith, G. A. Wells, M. P. Cranwell, and J. B. Ryan, Vet. Rec., 123, 638 (1988)   PUBMED
25 S. B. Prusiner, Proc. Natl. Acad. Sci. U S A., 95, 13363 (1998)   DOI   ScienceOn
26 B. Belosi, E. Gaggelli, R. Guerrini, H. Kozlowski, M. Luczkowski, F. M. Mancini, M. Remelli, D. Valensin, and G. Valensin, Chem. BioChem., 5, 349 (2004)
27 R. J. Slater, Experiments in Molecular Biology, Humana Press, New Jersey (1986)