[KSCI] Korea Science Citation Index Service

Characterization of Double-layer Optical Sensing Membranes for Dual Sensing of Dissolved Oxygen Concentrations and pH and Their Application to the On-line Monitoring of Fermentation Processes

Kim, Chun-Kwang (Department of Material and Biochemical Engineering, Chonnam National University,Research Center for Biophotonics, Chonnam National University,Center for Functional Nano Fine Chemicals, Chonnam National University)
Lee, Jong-Il (School of Applied Chemical Engineering, Chonnam National University,Research Institute for Catalysis, Chonnam National University,Research Center for Biophotonics, Chonnam National University,Center for Functional Nano Fine Chemicals, Chonnam National University)

Publication Information

Applied Chemistry for Engineering / v.20, no.4, 2009 , pp. 370-374 More about this Journal

Abstract

A double-layer optical sensing membrane has been fabricated to measure the concentration of dissolved oxygen (DO) and pH value simultaneously. (tris(4,7-diphenyl-1,10-phenanthroline (Rudpp) ruthenium(II)) as a DO sensitive dye has been mixed in the methyl trimethoxy silane (MTMS) sol-gel solution and coated onto one well in a 24-well microtiter plate. On the DO-sensing layer the GA (3-glycidoxypropyl trimethoxy silane (GPTMS), 3-aminopropyl trimethoxy silane (APTMS)) sol-gel solution mixed with 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt (HPTS) has been coated and used to measure pH values. The double-layer sensing membrane was affected by ionic strength and temperature. The double-layer sensing membrane for DO and pH has been applied to online monitor in microorganism cultivation processes and showed a good performance.

Keywords

double-layer; optical sensing membrane; fermentation monitoring; DO; pH;

Citations & Related Records

Times Cited By SCOPUS : 0

Reference

1	H. R. Kermis, Y. Kostov, and G. Rao, Analyst, 128, 1181 (2003) DOI ScienceOn
2	D. A. Nivens, M. V. Schiza, and S. M. Angel, Talan., 58, 543 (2002) DOI ScienceOn
3	C.-K. Kim, O.-J. Sohn, and J. I. Rhee, Proc. 2008 Spring KSBB meeting and Intern. Symp., 96, Chonbuk Nationnal University, Jeonju, Korea (2008)
4	F. Kensy, G. T. John, B. Hofmann, and J. Buchs, Bioprocess Biosyst. Eng., 75, 75 (2005)
5	S. Nagl and O. S. Wolfbeis, Analyst, 132, 507 (2007) DOI ScienceOn
6	A. S. Kocincova, S. M. Borisov, C. Krause, and O. S. Wolfbeis, Anal. Chem., 79, 8486 (2007) DOI ScienceOn
7	B. Zelelow, G. Khalil, G. Phelan, B. Carlson, M. Gouterman, J. B. Callis, and L. R. Dalton, Sens. Actuators B-Chem., 96, 304 (2003) DOI ScienceOn
8	S. M. Borisov, G. S. Vasylevska, C. Krause, and O. S. Wolfbeis, Adv. Funct. Mater., 16, 1536 (2006) DOI ScienceOn
9	S. M. Borisov, C. Krause, S. Arain, and O. S. Wolfbeis, Adv. Mater., 18, 1511 (2006) DOI ScienceOn
10	Y. J. Tang, D. Laidlaw, K. Gani, and J. D. Keasling, Biotechnol. Bioeng., 95, 176 (2006) DOI ScienceOn
11	Y. Amao and I. Okura, Sens. Actuators B-Chem., 88, 162 (2003) DOI ScienceOn
12	C.-K. Kim, O.-J. Sohn, and J. I. Rhee, Proc. Korean Sensors Conference, 77, Chosun University, Gwangju, Korea (2007)
13	M. E. Koese, B. F. Carrol, and K. S. Schanze, Langmuir, 21, 9121 (2005) DOI ScienceOn
14	K. P. McNamara, X. Li, A. D. Angela, and Z. Rosenzweing, Anal. Chim. Acta, 361, 73 (1998) DOI ScienceOn
15	P. Harms, Y. Kostov, J. A. French, M. Soliman, A. Anjanappa, A. Ram, and G. Rao, Biotechnol. Bioeng., 93, 6 (2006) DOI ScienceOn
16	A. C. Tizzard, J. H. Bergsma, and G. Lloyd-Jones, Biosen. & Bioelectro., 22, 759 (2006) DOI ScienceOn