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Enantioselective Kinetic Resolution of Racemic Styrene Oxide using Recombinant Marine Fish Epoxide Hydrolase of Mugil cephalus  

Choi, Sung Hee (Dept. of Food Science and Biotechnology, Kyungsung University)
Kim, Hee Sook (Dept. of Food Science and Biotechnology, Kyungsung University)
Lee, Eun Yeol (Dept. Of Chem. Eng., Kyung Hee University)
Publication Information
Applied Chemistry for Engineering / v.19, no.5, 2008 , pp. 491-496 More about this Journal
Abstract
The microsomal epoxide hydrolase gene (referred to as mMCEH) of Mugil cephalus was cloned by PCR, and then inserted to pColdI and pET-21b(+) vector, respectively. The recombinant E. coli possessing the recombinant plasmids exhibited the enantioperference toward (R)-styrene oxide. When enantioselective kinetic resolutions were conducted with 20 mM racemic styrene oxide, enantiopure (S)-styrene oxide was obtained with high enantiopurity more than 99% enantiomeric excess (ee) and 24.50% yield by using the recombinant E. coli harboring pET-21b(+)/mMCEH.
Keywords
epoxide hydrolase; Mugil cephalus; (S)-styrene oxide; enantioselective kinetic resolution;
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