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Preparation and Characterization of the Histidine-graft-Low Molecular Weight Water-Soluble Chitosan as a Gene Carrier  

Park, Jun-Kyu (Department of Polymer Science and Engineering, Sunchon National University)
Kim, Dong-gon (Department of Polymer Science and Engineering, Sunchon National University)
Choi, Changyong (Department of Polymer Science and Engineering, Sunchon National University)
Jang, Mi-Kyeong (Department of Polymer Science and Engineering, Sunchon National University)
Nah, Jae-Woon (Department of Polymer Science and Engineering, Sunchon National University)
Publication Information
Applied Chemistry for Engineering / v.18, no.6, 2007 , pp. 607-611 More about this Journal
Abstract
To improve transfection efficiency, we prepared histidine-low molecular weight water-soluble chitosan (LMWSC) having the potential to form complex with DNA as a cationic polymer. Histidine-LMWSC was synthesized by the esterification reaction and removing phthaloyl group. The histidine-LMWSC was characterized using FT-IR, $^1H$ NMR spectra. Histidine-LMWSC was complexed with plasmid DNA (pDNA) in various polymer/DNA (N/P) weight ratios, and the complex was identified using gel retardation assay. The particle sizes of the hisitidine-LMWSC/DNA complexes were measured on a DLS instrument by fixing the histidine-LMWSC/DNA weight ratio of 10/1. Owing to the utilization of a large excess amount of cationic LMWSC against anionic DNA, the particle size of histidine-LMWSC/DNA complexes was in the range of 100~200 nm. Therefore, histidine-LMWSC will be useful in the development of gene carriers.
Keywords
LMWSC; histidine; nanoparticle; gene therapy;
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