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A Study on the Immobilization of Lipase and Its Application for the Synthesis of Glycerides  

Chung, Dae-won (Department of Polymer Engineering, College of Engineering, Suwon University)
Song, Jhea-hyun (Department of Polymer Engineering, College of Engineering, Suwon University)
Paik, Min-jung (Department of Polymer Engineering, College of Engineering, Suwon University)
Publication Information
Applied Chemistry for Engineering / v.16, no.6, 2005 , pp. 731-736 More about this Journal
Abstract
In this paper, immobilization of lipase (Rhizomucor miehei, Rhm) onto ion-exchange resin pretreated with oleic acid and its application were studied. Immobilization efficiency was reached to 82% when weakly basic anion exchange resin, Duolite A-568, was used. Immobilized Rhm was stable in water, chloroform and hexane, however, unstable in alcoholic solvents. When immobilized Rhm was applied to the esterification reaction of glycerol and fatty acid, content of DG in the product mixture was ca. 80 mol% and 1,3-DG in total DG reached to 98%.
Keywords
immobilization; Rhizomucor miehei; oleic acid; diglyceride;
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