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Characteristics of Extraction of Daidzein and Genistein in Soybean Using Sub/Supercritical Fluids  

Choi, Du Young (Center for Advanced Bioseparation Technology and Department of Chemical Engineering, Inha University)
Zheng, Jinzhu (Center for Advanced Bioseparation Technology and Department of Chemical Engineering, Inha University)
Row, Kyung Ho (Center for Advanced Bioseparation Technology and Department of Chemical Engineering, Inha University)
Publication Information
Applied Chemistry for Engineering / v.16, no.5, 2005 , pp. 609-613 More about this Journal
Abstract
Daidzein and genistein were extracted from Korean soybean by supercritical $CO_2$ and sub/supercritical water. The extracted sample was analyzed by reversed-phase high performance liquid chromatography (RP-HPLC). The retention time, retention factor, column efficiency, column selectivity and resolution of aglycons were compared with the change in the temperature and pressure of supercritical fluid and ethanol concentration. The characteristics of extraction of daidzein and genistein were more affected by ethanol concentration using supercritical $CO_2$. The most desirable extraction yield was obtained by supercritical $H_2O$ with $400^{\circ}C$ and 250 bar. Generally, the extraction yield of aglycons increased over 10 times using supercritical $CO_2$ than sub/supercritical $H_2O$.
Keywords
characteristics; extraction; daidzein; genistein; sub/supercritical fluid;
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