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Preparation and Biocompatibility of 6-amino-6-deoxychitosan for Immobilization of Epidermal Growth Factor  

Son, Tae Il (Department of Biotechnology, University of Chung-Ang)
Park, Se Hoon (Department of Biotechnology, University of Chung-Ang)
Kang, Hahk Soo (Department of Biotechnology, University of Chung-Ang)
Jang, Eui Chan (Department of Orthopaedic Surgery, College of Medicine, University of Chung-Ang)
Publication Information
Applied Chemistry for Engineering / v.16, no.2, 2005 , pp. 226-230 More about this Journal
Abstract
Chitosan derivatives, 6-amino-6-deoxychitosan (6A6DC) was successively prepared as a reactive carbohydrate for the stabilization of epidermal growth factor (EGF) by the reactions of chitosan with tosyl chloride, sodium azide, and lithium aluminum tetrahydride. The structure of 6A6DC was confirmed by EA, FT-IR, $^1H-NMR$ and $^{13}C\{^1H\}-NMR$. The degree of substitution (ds) of amino groups in 6A6DC was determined to be 0.7. 6A6DC did not show any cytotoxicity on the normal human dermal fibroblast (NHDF) proliferation at least in the range tested (0.3 g/mL 600 g/mL) and was considered as a suitable material for the stabilization of EGF against proteolytic degradation due to its non-cytotoxicity and high reactivity.
Keywords
amino-polysaccharide; 6-amino-6-deoxychitosan; cytotoxicity;
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