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http://dx.doi.org/10.4489/KJM.20220028

Vertical Transmission of RNA Mycoviruses in Lentinula edodes  

Eunjin Kim (Forest Microbiology Division, Department of Forest Bio-Resources, National Institute of Forest Science)
Mi-Jeong Park (Forest Microbiology Division, Department of Forest Bio-Resources, National Institute of Forest Science)
Min-Jun Kim (Forest Microbiology Division, Department of Forest Bio-Resources, National Institute of Forest Science)
Yeun Sug Jeong (Forest Microbiology Division, Department of Forest Bio-Resources, National Institute of Forest Science)
Yeongseon Jang (Forest Microbiology Division, Department of Forest Bio-Resources, National Institute of Forest Science)
Kang-Hyeon Ka (Forest Microbiology Division, Department of Forest Bio-Resources, National Institute of Forest Science)
Publication Information
The Korean Journal of Mycology / v.50, no.4, 2022 , pp. 263-274 More about this Journal
Abstract
Lentinula edodes is an important commercial mushroom and there have been several reports of viral infections in L. edodes. Two mycoviruses (LeV-HKB and LeNSRV1) were detected in Sanbaekhyang (NIFoS 2778) and Taehyanggo (NIFoS 4317), the sawdust-cultivated commercial strains. The vertical transmission rates of the viruses were investigated by detecting the viruses in 80 monokaryotic strains derived from basidiospores isolated from the fruiting bodies of each strain. Most of the monokaryotic strains were infected with the virus and the two viruses showed different levels of meiotic stability, with LeV-HKB showing higher meiotic stability than LeNSRV1. Therefore, it seems that the vertical transmission mechanism of mycoviruses is different depending on the virus species. We also examined the mycelial growth rate of the monokaryotic strains and compared the growth rate according to virus infection status. Although there was no statistically significant correlation between viral infection and mycelial growth rate, we found that the average growth rate was reduced by additional virus infection. We expect our data to contribute to a greater understanding of the mechanism of the vertical transmission of mycoviruses, and promoting breeding using virus-free monokaryotic strains.
Keywords
Basidiospore; Lentinula edodes; Mycovirus; RT-PCR; Transmission;
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