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http://dx.doi.org/10.4489/KJM.20220002

Production and Characterization of the Beneficial β-glucuronidase Inhibitor from non pathogenic wild yeast, Candida oleophila WP5-19-1, and its effects on gut microbes  

Seon-Jeong Park (Department of Biomedicine and Biotechnology, Paichai University)
Jong-Soo Lee (Department of Biomedicine and Biotechnology, Paichai University)
Publication Information
The Korean Journal of Mycology / v.50, no.1, 2022 , pp. 31-40 More about this Journal
Abstract
We aimed to produce a potent β-glucuronidase inhibitor from wild yeast that could inactivate toxic substances in the colon. Culture supernatants and cell-free extracts of non-pathogenic wild yeasts were prepared and their β-glucuronidase inhibitory activities were measured. Cell-free extract from Candida oleophila WP5-19-1 showed the highest β-glucuronidase inhibitory activity (49.0%). The β-glucuronidase inhibitor was maximally produced (IC50 value; 8.4 mg) when C. oleophila WP5-19-1 was cultured in potato dextrose medium containing 5% dextrose (initial pH; 6.0) at 30℃ for 24 hours. β-glucuronidase inhibitor of C. oleophila WP5-19-1 was partially purified by trypsin hydrolysis, ultrafiltration (3 kDa), and Sephadex G-50 filtration. The partially purified β-glucuronidase inhibitor was stable from 30℃ to 60℃ and at pH 6.0 9.0, and showed residual inhibitory activity of about 80%.
Keywords
${\beta}$-glucuronidase inhibitor; Candida oleophila WP5-19-1; Non-pathogenic wild yeast; Partial purification; Probiotics;
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Times Cited By KSCI : 6  (Citation Analysis)
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