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http://dx.doi.org/10.4489/KJM.20210019

Characteristics of Fibrinolytic Enzymes of Bacillus licheniformis CY-24 Isolated from Button Mushroom Compost  

Min, Gyeong-Jin (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA)
Park, Hea-sung (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA)
Lee, Een-ji (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA)
Lee, Chan-Jung (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA)
Publication Information
The Korean Journal of Mycology / v.49, no.2, 2021 , pp. 199-209 More about this Journal
Abstract
The present study was performed to improve the technique used for fermenting the mushroom growth medium. Taxonomic analysis of 16S rDNA sequence from the predominant Bacillus strain CY-24 isolated during the fermentation phase of the rice straw medium identified it as Bacillus licheniformis. In addition, the growth environment of B. licheniformis was also examined in this study, which revealed the optimal growth temperature and pH to be 30 ℃ and 6.0, respectively. This study also revealed that carboxymethyl cellulase (CMCase) and polygalacturonase (PGase) enzymes isolated from B. licheniformis achieved their maximal activities at 50 ℃ and 60 ℃ respectively. Furthermore, the study confirmed that the two enzymes, i.e., CMCase and PGase in B. licheniformis are stable at temperatures above 60 ℃. The present study thus demonstrates that B. licheniformis CY-24 possesses excellent enzymatic properties. It also reveals that the action of enzymes during the production of growth mediums used for the cultivation of mushrooms is closely associated with the promotion of fermentation and softening of the rice straw. Overall, this study provides elementary information regarding the role of B. licheniformis enzymes during growth medium fermentation for Agaricus bisporus cultivation.
Keywords
Agaricus bisporus; Cultivation; Fermentation techniques; Media composition; Mushroom;
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1 Rao M, Seeta R, Deshpande V. Effect of pretreatment on the hydrolysis of cellulose by Penicillium funiculosum cellulase and recovery of enzyme. Biotechnol Bioeng 1983;25:1863-71.   DOI
2 Elshafei AM, Vega JL, Klasson KT, Clausen EC, Gaddy JL. The saccharification of corn stover by cellulase from Penicillium funiculosum. Bioresour Technol 1991;35:73-80.   DOI
3 Regine MD, Ptak M, Peypouxb F, Michel G. Pore-forming properties of iturin A, a lipopeptide antibiotic. Biochim Biophys Acta 1985;815:405-9.   DOI
4 Schallmey M, Singh A, Ward OP. Developments in the use of Bacillus species for industrial production. Can J Microbiol 2004;50:1-17.   DOI
5 Shin PG, Cho SJ. Cellulase and xylanase activity of compost-promoting bacteria Bacillus sp. SJ21. KSSSF 2011;44:836-40.
6 Saitou N, Nei M. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406-25.
7 Palleroni NJ, Krieg NR, Holt JG. Bergey's manual of systematic bacteriology. Baltimore: The Willian and Wilkins. Co; 1984. p. 141-219.
8 Thompson JD, Gibson TJ, Higgins DG. Multiple sequence alignment using ClustalW and ClustalX. Curr Protoc Bioinformatics 2003;1:2-3.
9 Rey M, Ramaiya P, Nelson BA, Brody-Karpin SD, Zaretsky EJ, Tang M, Berka RM. Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species. Genome Biol 2004;5:1-12.
10 Min KJ, Kim JK, Kwak AM, Kong WS, Oh YH, Kang HW. Genetic diversity of Agaricus bisporus strains by PCR polymorphism. Kor J Mycol 2014;42:1-8.   DOI
11 Doi RH, Kosugi A. Cellulosomes: Plant-cell-wall-degrading enzyme complexes. Nat Rev. Microbiol 2004;2:541-51.   DOI
12 Lee JH, Choi SH. Xylanase production by Bacillus sp. A-6 isolated from rice bran. J Microbiol Biotechnol 2006;16:1856-61.
13 Gerrits JPG, Bels-Koning HC, Muller FM. Changes in compost constituents during composting, pasteurization and cropping. J Mushrooms 1967;6:225-43.
14 Rosenberg SL. Cellulose and lignocellulose degradation by thermophilic and thermotolerant fungi. Mycologia 1978;70:1-13.   DOI
15 Taguchi F, Yamada K, Hasegawa K, Taki-Saito T, Hara K. Continuous hydrogen production by Clostridium sp. strain no. 2 from cellulose hydrolysate in an aqueous two-phase system. J Ferment Bioeng 1996;82:80-83.   DOI
16 Kim ES, Lee HJ, Bang WG, Choi IG, Kim KH. Functional characterization of a bacterial expansin from Bacillus subtilis for enhanced enzymatic hydrolysis of cellulose. Biotechnol Bioeng 2009;102:1342-53.   DOI
17 Kim JY, Heo SH, Hong JH. Isolation and characterization of an alkaline cellulase produced by alkalophilic Bacillus sp. HSH-810. J Microbiol Biotechnol 2004;40:139-46.
18 Milijasevic-Marcic S, Stepanovic M, Todorovic B, Duduk B, Stepanovic J, Rekanovic E, Potocnik I. Biological control of green mould on Agaricus bisporus by a native Bacillus subtilis strain from mushroom compost. Eur J Plant Pathol 2017;148:509-19.   DOI
19 Lin L, Kan X, Yan H, Wang D. Characterization of extracellular cellulose-degrading enzymes from Bacillus thuringiensis strains. Electron J Biotechnol 2012;15:1-7.
20 Sun S, Zhang Y, Liu K, Chen X, Jiang C, Huang M, Li C. Insight into biodegradation of cellulose by psychrotrophic bacterium Pseudomonas sp. LKR-1 from the cold region of China: Optimization of cold-active cellulase production and the associated degradation pathways. Cellulose 2020;27:315-33.   DOI
21 Kim DJ, Shin HJ, Min BH, Yoon KH. Isolation of a thermophilic Bacillus sp. producing the thermostable cellulase-free xylanase, and properties of the enzyme. J Microbiol Biotechnol 1995;23:304-10.   DOI
22 Nakano MM, Marahiel MA, Zuber P. Identification of a genetic locus required for biosynthesis of the lipopeptide antibiotic surfactin in Bacillus subtilis. J Bacteriol 1988;170:5662-8.   DOI
23 Waghmare SR, Ghosh JS. Chitobiose production by using a novel thermostable chitinase from Bacillus licheniformis strain JS isolated from a mushroom bed. Carbohydr Res 2010;345:2630-35.   DOI
24 Reasoner DJ, Geldreich EE. A new medium for the enumeration and subculture of bacteria from potable water. Appl Environ Microbiol 1985;49:1-7.   DOI
25 Miller GL, Blum R, Glennon WE, Burton AL. Measurement of carboxymethyl cellulase activity. Anal Biochem 1960;1:127-32.   DOI
26 Karim A, Nawaz MA, Aman A, Qader SAU. Hyper production of cellulose degrading endo (1,4) β-d-glucanase from Bacillus licheniformis KIBGE-IB2. J Radiat Res Appl Sci 2015;8:160-5.   DOI
27 Choi WH, Choi YS, Jang KY, Yoon MH. Xylanase properties of Bacillus subtilis AB-55 isolated from waste mushroom bed of Agaricus bisporus. Korean J Agric Sci 2012;39:255-61.   DOI
28 Roongsawang TT. Kameyama M. Haruki M. Morikawa. Isolation and characterization of a halotolerant Bacillus subtilis BBK-1 which produces three kinds of lipopeptides: Bacillomycin L, plipastain and surfactin. Extremophiles 2002;6:499-506.   DOI
29 Kim MJ, Lim SJ, Kang DK. Isolation of a Bacillus licheniformis DK42 producing cellulase and xylanase, and properties of the enzymes. J Anim Sci Technol 2008;50:429-36.   DOI
30 Vijayalakshmi S, Ranjitha J, Rajeswari VD. Enzyme production ability by Bacillus subtilis and Bacillus licheniformis-A comparative study. Asian J Pharm Clin Res 2013;6:29-32.
31 Lee CJ, Yu BK, Park HS, Lee EJ, Min GJ. Characteristics of the media under a self-propelled compost turner in button mushroom cultivation. J Mushrooms 2020;18:274-9.
32 Lim SH, Lee YH, Kang HW. Optimal extraction and characteristics of lignocellulytic enzymes from various spent mushroom composts. Mycobiology 2013;41:160-6.
33 Lee CJ, Yoo YM, Moon JW, Cheong JC, Kong WS, Kim YG, Lee BE, Yoon MH, Sa TM. Characteristics and distribution of microorganisms in a rice straw compost for cultivation of button mushrooms (Agaricus bisporus). Kor J Mycol 2017;45:43-53.   DOI
34 Kim TI, Han JD, Jeon BS, Ha SW, Yang CB, Kim MK. Isolation and characterization of Bacillus subtilis CH-10 secreting cellulase from cattle manure. Kor J Mycol 1999;35:277-82.
35 Reese ET. Enzymatic hydrolysis of cellulose. Appl Microbiol 1956;4:39-45.   DOI
36 Halliwell G, Riaz M. The formation of short fibres from native cellulose by components of Trichoderma koningii cellulase. Biochem J 1970;116:35-42.   DOI
37 Gomaa EZ. Chitinase production by Bacillus thuringiensis and Bacillus licheniformis: Their potential in antifungal biocontrol. Microbiology 2012;50:103-11.
38 Lee BE, Lee CJ, Yoon MH, Kim YG, Lee BJ. New cultivation method of button mushroom (Agaricus bisporus) utilizing mixture of sawdust and chicken manure. J Mushrooms 2016;14:179-83.   DOI
39 Bark SW, Kim WR, Kim MJ, Kang BK, Pak WM, Kim BR, Ahn DH. Optimization and characterization of conditions for cellulose-degrading crude enzymes produced by Cellulophaga lytica PKA 1005. J Microbiol Biotechnol 2014;42:18-24.
40 de Boer AS, Priest F, Diderichsen B. On the industrial use of Bacillus licheniformis: A review. Appl Microbiol Biotechnol 1994;40:595-8.   DOI