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http://dx.doi.org/10.4489/KJM.20200038

Development and Application of a Cleaved Amplified Polymorphic Sequence Marker for Discriminating A Mating Type Alleles of Lentinula edodes  

Park, Mi-Jeong (Division of Special Forest Products, Department of Forest Bioresources, National Institute of Forest Science)
Ryoo, Rhim (Division of Special Forest Products, Department of Forest Bioresources, National Institute of Forest Science)
Jang, Yeongseon (Division of Special Forest Products, Department of Forest Bioresources, National Institute of Forest Science)
Ka, Kang-Hyeon (Division of Special Forest Products, Department of Forest Bioresources, National Institute of Forest Science)
Publication Information
The Korean Journal of Mycology / v.48, no.4, 2020 , pp. 389-396 More about this Journal
Abstract
Lentinula edodes is one of the most widely consumed edible mushrooms in Korea. Mating in L. edodes is regulated by a tetrapolar system, and two unlinked genetic loci, A and B, are known to be major determinants of the mating types, as reported in other heterothallic basidiomycetes. The A locus of L. edodes encodes a pair of homeodomain (HD) transcription factors. The highly variable N-termini of these HD transcription factors contribute to the diversity among the A mating types. In this study, we developed a cleaved amplified polymorphic sequence (CAPS) marker to discriminate 11 different A mating type alleles predominant among both cultivated and wild strains. Amplification of the variable region of the A locus followed by digestion with HaeIII and EcoRI restriction enzymes enabled successful discrimination among the 11 A mating type alleles. We also evaluated the applicability of this method in the identification of two A mating types of a dikaryotic strain.
Keywords
Cleaved amplified polymorphic sequence (CAPS) marker; Lentinula edodes; Mating type; Mushroom breeding; Restriction enzyme;
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Times Cited By KSCI : 6  (Citation Analysis)
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