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http://dx.doi.org/10.4489/KJM.20200032

Identification of Sphaerulina azaleae on Korean Azalea in Korea Based on Morphological Characteristics and Multilocus Sequence Typing  

Choi, In-Young (Department of Agricultural Biology, Jeonbuk National University)
Choi, Young-Joon (Department of Biology, Kunsan National University)
Lee, Kui-Jae (Division of Biotechnology, College of Environmental and Bioresource Sciences, Jeonbuk National University)
Ju, Ho-Jong (Department of Agricultural Biology, Jeonbuk National University)
Cho, Seong-Wan (Department of Pharmaceutics & Biotechnology, Konyang University)
Shin, Hyeon-Dong (Division of Environmental Science and Ecological Engineering, Korea University)
Publication Information
The Korean Journal of Mycology / v.48, no.3, 2020 , pp. 329-335 More about this Journal
Abstract
From 2008 to 2017, Korean azalea (Rhododendron yedoense f. poukhanense) showing angular, necrotic leaf spots were found in Jeju and Hongcheon, Korea. The lesions occurred frequently, detracting from the beauty of the glossy green leaves of the plant and causing premature defoliation. Therefore, to identify the fungus associated with the lesions, morphological characterization and molecular phylogenetic analysis of actin (Act), translation elongation factor 1-alpha (EF), internal transcribed spacer (ITS), 28S nrDNA (LSU), and RNA polymerase II encoding the second largest subunit (RPB2) of the two representative isolates were performed. The phylogenetic tree inferred from the neighbor-joining method showed the isolates clustering in the Sphaerulina azaleae group. Therefore, the fungus associated with the angular leaf spots on the Korean azalea was identified as Sphaerulina azaleae.
Keywords
Angular leaf spot; Korean azalea; Rhododendron yedoense f. poukhanense; Sphaerulina azaleae;
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