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http://dx.doi.org/10.4489/KJM.20200002

Optimal Culture Conditions for Penicillium rubefaciens NNIBRFG5039 Possessing Antimicrobial Activity  

Hwang, Hye Jin (Fungi Research Team, Microbial Research Department, Nakdonggang National Institute of Biological Resources (NNIBR))
Mun, Hye Yeon (Fungi Research Team, Microbial Research Department, Nakdonggang National Institute of Biological Resources (NNIBR))
Hwang, Buyng Su (Animal&Plant Utilization Team, Animal&Plant Research Department, Nakdonggang National Institute of Biological Resources (NNIBR))
Nam, Young Ho (Environmental Microbiology Research Team, Microbial Research Department, Nakdonggang National Institute of Biological Resources (NNIBR))
Chung, Eu Jin (Environmental Microbiology Research Team, Microbial Research Department, Nakdonggang National Institute of Biological Resources (NNIBR))
Publication Information
The Korean Journal of Mycology / v.48, no.1, 2020 , pp. 15-27 More about this Journal
Abstract
In screening for antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA) KCCM 40510 and Bacillus cereus KCTC 3624, NNIBRFG5039 was isolated from the air in Sangju-si, Gyeongsangbuk-do. Based on a high sequence similarity of the internal transcribed spacer (ITS) region, NNIBRFG5039 was determined to be closely related to Penicillium rubefaciens CBS 139145. The optimal media, initial pH, and temperature for mycelial growth and antimicrobial activity of P. rubefaciens NNIBRFG5039 were determined as follows: potato dextrose broth (PDB), pH 6.5, and 30℃, respectively. Under the optimal culture conditions, maximum mycelial growth (12.4 g L-1) and antibacterial activity (7.5 mm zone of inhibition against MRSA KCCM 40510, and 5.0 mm zone of inhibition against B. cereus KCTC 3624) were observed in a 5 L stirred-tank fermenter. We also isolated the antimicrobial compound from an ethyl acetate fraction, and its chemical structure was identified as (S)-6-hydroxymellein (1) by ESI-MS, 1H-NMR, and 13C-NMR. Consequently, the extract from P. rubefaciens NNIBRFG5039 may be used in functional materials for antimicrobial-related applications.
Keywords
Antimicrobial activity; Methicillin-resistant Staphylococcus aureus; Penicillium rubefaciens; (S)-6-hydroxymellein;
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