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http://dx.doi.org/10.4489/KJM.20190039

Production of Anti-dementia Acetylcholinesterase Inhibitor from Pleurotus ostreatus (Heuktari) and Inhibitory Effect on PC12 Neuron Apoptosis  

Han, Sang-Min (Department of Biomedicinal Science and Biotechnology, Paichai University)
Kim, Ji-Yoon (Department of Biomedicinal Science and Biotechnology, Paichai University)
Lee, Jong-Soo (Department of Biomedicinal Science and Biotechnology, Paichai University)
Publication Information
The Korean Journal of Mycology / v.47, no.4, 2019 , pp. 337-346 More about this Journal
Abstract
To develop a new antidementia acetycholinesterase (AChE) inhibitor from edible mushrooms, the inhibitory effects on AChE of water and ethanol extracts from various edible mushrooms were measured. Among the tested compounds, 70% ethanol extracts from Tremella fuciformis showed the highest AChE inhibitory activity, at 25.3% (IC50: 9.9 mg). Water extracts from the fruiting body of Pleurotus ostreatus (Heuktari) showed AChE inhibitory activity of 20.2% (IC50: 12.4 mg). However, the yield (40.8%) from Pleurotus ostreatus (Heuktari) was higher than that from Tremella fuciformis (5.0%). Therefore, we selected Pleurotus ostreatus (Heuktari) as the most promising candidate for a mushroom containing anti-dementia AChE inhibitors. The AChE inhibitor from Pleurotus ostreatus (Heuktari) was optimally extracted when its fruiting body was treated with water for 6 h at 30℃. The anti-dementia effects of the partially purified AChE inhibitor from Pleurotus ostreatus (Heuktari) were observed in PC12 nerve cells.
Keywords
Acetylcholinesterase inhibitor; Anti-dementia; Nerve cell; PC12; Pleurotus ostreatus (Heuktari); Water extracts;
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Times Cited By KSCI : 16  (Citation Analysis)
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