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http://dx.doi.org/10.4489/KJM.20180050

Characterization of Pyrenophora avenae Isolated from Discolored Black Oat Seeds in Korea  

Choi, Jung-Hye (Microbial Safety Team, National Institute of Agricultural Sciences)
Kim, Jeomsoon (Crop Protection Division, National Institute of Agricultural Sciences)
Ham, Hyeonheui (Microbial Safety Team, National Institute of Agricultural Sciences)
Lee, Theresa (Microbial Safety Team, National Institute of Agricultural Sciences)
Nah, Ju-Young (Microbial Safety Team, National Institute of Agricultural Sciences)
Choi, Hyo-Won (Crop Protection Division, National Institute of Agricultural Sciences)
Lee, Young Kee (Crop Protection Division, National Institute of Agricultural Sciences)
Hong, Sung Kee (Crop Protection Division, National Institute of Agricultural Sciences)
Publication Information
The Korean Journal of Mycology / v.46, no.4, 2018 , pp. 459-466 More about this Journal
Abstract
In January 2017, discolored black oat seeds were found in the storage depot of a farmhouse in Jeongeup. Pyrenophora sp. was detected in 45% of the oat seeds surveyed. All Pyrenophora isolates obtained from the seeds were identified as Pyrenophora avenae based on the sequences of internal transcribed spacer (ITS) rDNA regions and glyceraldehyde 3-phosphate dehydrogenase (GPDH) gene and validated by morphological and cultural characterization. A phylogenetic tree constructed using the ITS and GPDH sequences showed that the Korean isolates of P. avenae comprise of four genetically distinct groups. Pathogenicity test validated that the fungus is an infectious agent responsible for discolored black seeds and leaf blotch in oat plants. This is the first study report that P. avenae causes leaf blotch disease of oat in Korea.
Keywords
Black discolored seeds; Leaf blotch; Oat; Pyrenophora avenae;
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