Browse > Article
http://dx.doi.org/10.4489/KJM.20180015

First Report on Four Novel Endophytic Fungal Species Isolated from Leaves of Juniperus chinensis var. sargentii in Korea  

Park, Hyeok (Department of Biology Education, Korea National University of Education)
Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
Publication Information
The Korean Journal of Mycology / v.46, no.2, 2018 , pp. 114-121 More about this Journal
Abstract
We isolated endophytic fungi from the leaves of the conifer Juniperus chinensis var. sargentii inhabiting Mt. Hallasan. The fungal isolates were identified based on phylogenetic analysis using nucleotide sequences of the internal transcribed spacer region, large subunit region of ribosomal DNA, translation elongation factor, and ${\beta}$-tubulin region. In this study, we confirmed four species of unreported endophytic fungi in Korea, Allantophomopsis lycopodina, Epicoleosporium ramularioides, Kabatina juniperi, and Seiridium podocarpi. We describe their morphological characteristics and the results of the phylogenetic analysis.
Keywords
Allantophomopsis lycopodina; Epicoleosporium ramularioides; Kabatina juniperi; Seiridium podocarpi;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Hata K, Futai K, Tsuda M. Seasonal and needle age-dependent changes of the endophytic mycobiota in Pinus thunbergii and Pinus densiflora needles. Can J Bot 1998;76:245-50.
2 Gardes M, Bruns TD. ITS primers with enhanced specificity for basidiomycetes‐ application to the identification of mycorrhizae and rusts. Mol Ecol 1993;2:113-8.   DOI
3 Moncalvo JM, Lutzoni FM, Rehner SA, Johnson J, Vilgalys R. Phylogenetic relationships of agaric fungi based on nuclear large subunit ribosomal DNA sequences. Syst Biol 2000;49:278-305.   DOI
4 Glass NL, Donaldson GC. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol 1995; 61:1323-30.
5 Rehner SA, Buckley E. A Beauveria phylogeny inferred from nuclear ITS and EF1-${\alpha}$ sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 2005;97:84-98.
6 Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 2013;30:2725-9.   DOI
7 Videira SI, Groenewald JZ, Braun U, Shin HD, Crous PW. All that glitters is not Ramularia. Stud Mycol 2016;83:49-163.   DOI
8 Schneider R, von Arx JA. Two new fungi causing twig dieback: Kabatina thujae gen. et sp. nov. and K. juniperi sp. nov. J Phytopathol 1966;57:176-82.   DOI
9 Petrini O, Carroll G. Endophytic fungi in foliage of some Cupressaceae in Oregon. Can J Bot 1981;59:629-36.   DOI
10 Carris LM. Cranberry black rot fungi: Allantophomopsis cytisporea and Allantophomopsis lycopodina. Can J Bot 1990;68:2283-91.   DOI
11 Debbab A, Aly AH, Proksch P. Bioactive secondary metabolites from endophytes and associated marine derived fungi. Fungal Divers 2011;49:1.   DOI
12 Shiono Y, Yokoi M, Koseki T, Murayama T, Aburai N, Kimura K. Allantopyrone A, a new ${\alpha}$-pyrone metabolite with potent cytotoxicity from an endophytic fungus, Allantophomopsis lycopodina KS-97. J Antibiot 2010;63:251-3.   DOI
13 Crous PW, Shivas RG, Quaedvlieg W, van der Bank M, Zhang Y, Summerell BA, Guarro J, Wingfield MJ, Wood AR, Alfenas AC, et al. Fungal Planet description sheets: 214-280. Persoonia 2014;32:184-306.   DOI
14 Carroll G. Fungal endophytes in stems and leaves: from latent pathogen to mutualistic symbiont. Ecology 1988;69:2-9.   DOI
15 Schulz B, Boyle C, Draeger S, Rommert AK, Krohn K. Endophytic fungi: a source of novel biologically active secondary metabolites. Mycol Res 2002;106:996-1004.   DOI
16 Tan RX, Zou WX. Endophytes: a rich source of functional metabolites. Nat Prod Rep 2001;18:448-59.   DOI