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http://dx.doi.org/10.4489/KJM.20170004

Characterization of Wild Yeasts Isolated from Leaves Obtained from Mt. Daedun and Mt. Chilgap, Korea  

Han, Sang-Min (Department of Biomedicinal Science and Biotechnology, Paichai University)
Lee, Sang-Yeop (Department of Biomedicinal Science and Biotechnology, Paichai University)
Kim, Ha-Kun (Department of Biomedicinal Science and Biotechnology, Paichai University)
Lee, Jong-Soo (Department of Biomedicinal Science and Biotechnology, Paichai University)
Publication Information
The Korean Journal of Mycology / v.45, no.1, 2017 , pp. 31-42 More about this Journal
Abstract
Several yeast strains were isolated from leaves collected from Mt. Daedun and Mt. Chilgap in Korea. A total of 79 strains of 32 species were isolated from 44 leaves obtained from Mt. Daedun. Cryptococcus spp., including Cryptococcus magnus, were found to be dominant, while 16 strains of Aureobasidium pullulans were isolated. Among the strains isolated from Mt. Daedun, Asterotremella humicola JSL502, Barnettozyma californica JSL503, Bullera unica JSL501, Bulleromyces albus JSL505, and Candida vaccinii JSL507 are considered rare yeast species in Korea. All of these rare strains, except for Bulleromyces albus JSL505, formed ascospores. Bullera unica JSL501 was able to grow in yeast extract-peptone-dextrose (YPD) medium containing 10% NaCl. Twenty-five yeast strains of 18 species were isolated from 43 leaves obtained from Mt. Chilgap. Six strains of Pseudozyma spp., including three strains of Pseudozyma aphidis, were isolated in the dormant state. Of these strains, the occurrence of Rhodotorula phylloplana JSL515, Rhodotorula pinicola JSL516, Schwanniomyces vanrijiae JSL517, and Sporisorium loudetiae JSL518 is rare in Korea. Only Sporisorium loudetiae JSL518 formed ascospores, while Rhodotorula pinicola JSL516 and Sporisorium loudetiae JSL518 formed pseudomycelia. The halophilic yeast Schwanniomyces vanrijiae JSL517 was isolated and observed to grow in YPD medium containing 5% NaCl.
Keywords
Chilgap Mt.; Daedun Mt.; Isolation; Leaves; Yeasts;
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Times Cited By KSCI : 13  (Citation Analysis)
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1 Lee JS, Yi SH, Kwon SJ, Ahn C, Yoo JY. Enzyme activities and physiological functionality of yeasts from traditional Meju. Korean J Appl Microbiol Biotechnol 1997;25:448-53.
2 Min JH, Kim YH, Kim JH, Choi SY, Lee JS, Kim HK. Comparison of microbial diversity of Korean commercial Makgeolli showing high ${\beta}$-glucan content and high antihypertensive activity, respectively. Mycobiology 2012;40:138-41.   DOI
3 Min JH, Ryu JJ, Kim HK, Lee JS. Isolataion and identification of yeasts from wild flowers in Gyejoksan, Oseosan and Baekamsan of Korea. Kor J Mycol 2013;41:47-51.   DOI
4 Golubev VI, Nakase T. Mycocinogeny in Bullera genus: killer activity of Bullera unica and intragenus killer-sensitive relationships. Mikrobiologiia 1998;67:225-30.
5 Nakase T. Expanding world of ballistosporous yeasts: distribution in the phyllosphere, systematics and phylogeny. J Gen Appl Microbiol 2000;46:189-216.   DOI
6 Moriyama D, Kaino T, Yajima K, Yanai R, Ikenaka Y, Hasegawa J, Washida M, Nanba H, Kawamukai M. Cloning and characterization of decaprenyl diphosphate synthase from three different fungi. Appl Microbiol Biotechnol 2017;101:1559-71.   DOI
7 Sampaio JP, Inácio J, Fonseca A, Gadanho M, Spencer-Martins I, Scorzetti G, Fell JW. Auriculibuller fuscus gen. nov., sp. nov. and Bullera japonica sp. nov., novel taxa in the Tremellales. Int J Syst Evol Microbiol 2004;54:987-93.   DOI
8 Sipiczki M. Starmerella syriaca f.a., sp. nov., an osmotolerant yeast species isolated from flowers in Syria. Antonie Van Leeuwenhoek 2015;107:847-56.   DOI
9 Janisiewicz WJ, Jurick WM 2nd, Peter KA, Kurtzman CP, Buyer JS. Yeasts associated with plums and their potential for controlling brown rot after harvest. Yeast 2014;31:207-18.   DOI
10 Zhou ZX, Jiang H, Yang C, Yang MZ, Zhang HB. Microbial community on healthy and diseased leaves of an invasive plant Eupatorium adenophorum in southwest China. J Microbiol 2010;48:139-45.   DOI
11 Vaca I, Faundez C, Maza F, Paillavil B, Hernandez V, Acosta F, Levican G, Martinez C, Chavez R. Cultivable psychrotolerant yeasts associated with Antarctic marine sponges. World J Microbiol Biotechnol 2013;29:183-9.   DOI
12 Maksimova IA, Glushakova AM, Kachalkin AV, Chernov IY, Panteleeva SN, Reznikova ZI. Yeast communities of Formica aquilonia colonies. Microbiology 2016;85:124-9.   DOI
13 Hyun SH, Min JH, Kim SA, Lee JS, Kim HK. Yeasts associated with fruits and blossoms collected from Hanbat arboretum, Daejeon, Korea. Kor J Mycol 2014;42:178-82.   DOI
14 Hyun SH, Mun HY, Lee HB, Kim HK, Lee JS. Isolation of yeasts from wild flowers in Gyonggi-do province and Jeju island in Korea and the production of anti-gout xanthine oxidase inhibitor. Korean J Microbiol Biotechnol 2013;41:383-90.   DOI
15 Hyun SH, Min JH, Lee HB, Kim HK, Lee JS. Isolation and diversity of yeasts from wild flowers in Ulleungdo and Yokjido, Korea. Kor J Mycol 2014;42:28-33.   DOI
16 Hyun SH, Lee JG, Park WJ, Kim HK, Lee JS. Isolation and diversity of yeasts from fruits and flowers of orchard in Sinam-myeon of Yesan-gun, Chungcheongnam-do, Korea. Kor J Mycol 2014;42:21-7.   DOI
17 Hyun SH, Han SM, Lee JS. Isolation and physiological functionality of yeasts from wild flowers in Seonyudo of Gogunsanyeoldo, Jeollabuk-do, Korea. Kor J Mycol 2014;42:201-6.   DOI
18 Han SM, Hyun SH, Lee JS. Isolation and identification of yeasts from wild flowers in Deogyu mountain and their physiological functionalities. Kor J Mycol 2015;43:47-52.   DOI
19 Ingold CT. Unusual teliospore germination in Ustilago triodiae and Sporisorium loudetiae-pedicellatae. Mycol Res 1999;103:137-40.   DOI
20 Han SM, Hyun SH, Lee HB, Lee HW, Kim HK, Lee JS. Isolation and identification of yeasts from wild flowers collected around Jangseong lake in Jeollanam-do, republic of Korea, and characterization of the unrecorded yeast Bullera coprosmaensis. Mycobiology 2015;43:266-71.   DOI
21 Han SM, Han JW, Bae SM, Park WJ, Lee JS. Isolation and identification of wild yeasts from soils of paddy fields in Daejeon metropolitan city and Chungcheongnam-do, Korea. Kor J Mycol 2016;44:1-7.   DOI
22 Han SM, Lee JS. Isolation and identification of wild yeasts from soils of an herb park in Seoul metropolitan city and characteristics of unrecorded yeasts. Kor J Mycol 2016;44:108-12.
23 Han SM, Kim HK, Lee HB, Lee JS. Isolation and identification of wild yeasts from freshwaters and soils of Nakdong and Yeongsan river, Korea, with characterization of two unrecorded yeasts. Kor J Mycol 2016;44:350-4.
24 Min JH, Lee HB, Lee JS, Kim HK. Identification of yeasts isolated from wild flowers collected in coast areas of Korea based on the 26S rDNA sequences. Kor J Mycol 2013;41:185-91.   DOI
25 Cho DH, Kim JM. Biodiversities and fungal resources of Mt. Daedun provincial park. Korean J Plant Res 2003;16:119-20.
26 Cadete RM, Melo MA, Dussán KJ, Rodrigues RC, Silva SS, Zilli JE, Vital MJ, Gomes FC, Lachance MA, Rosa CA. Diversity and physiological characterization of D-xylose-fermenting yeasts isolated from the Brazilian Amazonian Forest. PLoS One 2012;7:e43135.   DOI
27 Drumonde-Neves J, Franco-Duarte R, Lima T, Schuller D, Pais C. Yeast biodiversity in vineyard environments is increased by human intervention. PLoS One 2016;11:e0160579.   DOI
28 Morais CG, Cadete RM, Uetanabaro AP, Rosa LH, Lachance MA, Rosa CA. D-xylosefermenting and xylanase-producing yeast species from rotting wood of two atlantic rainforest habitats in brazil. Fungal Genet Biol 2013;60:19-28.   DOI