Browse > Article
http://dx.doi.org/10.4489/KJM.2016.44.1.61

Sclerotium Rot of Cowpea (Vigna sinensis King) Caused by Sclerotium rolfsii  

Kwon, Jin-Hyeuk (Gyeongsangnam-do Agricultural Research and Extension Services)
Kang, Dong-Wan (Gyeongsangnam-do Agricultural Research and Extension Services)
Han, Inyoung (Gyeongsangnam-do Agricultural Research and Extension Services)
Choi, Yong-Jo (Gyeongsangnam-do Agricultural Research and Extension Services)
Lee, Sang-Dae (Gyeongsangnam-do Agricultural Research and Extension Services)
Son, Daeyoung (Department of Plant Medicine, Gyeongsang National University)
Publication Information
The Korean Journal of Mycology / v.44, no.1, 2016 , pp. 61-63 More about this Journal
Abstract
Sclerotium rot disease on cowpea (Vigna sinensis King) was observed in the exhibition field of Gyeongsangnam-do Agricultural Research and Extension Services in September 2015. Lesions were covered by white mycelial mats, and numerous sclerotia were formed on the stem near the soil line. The sclerotia were globoid in shape, 1~3 mm in size and white to brown in color. The optimum temperature for mycelial growth and sclerotia formation on potato dextrose agar (PDA) was $30^{\circ}C$, with the hyphal width of $4{\sim}8{\mu}m$. For molecular identification, the complete internal transcribed spacer (ITS) rDNA region of the causal fungus was sequenced and analyzed. Based on the mycological characteristics, ITS rDNA sequence analysis, and pathogenicity test, this fungus was identified as Sclerotium rolfsii. This is the first report of sclerotium rot on cowpea caused by S. rolfsii in Korea.
Keywords
Cowpea; Sclerotium rolfsii; Sclerotium rot; Vigna sinensis;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Lee TB. Coloured flora of Korea. Hyangmoonsa: Seoul; 2003.
2 Korean Society of Plant Pathology. List of plant diseases in Korea. 5th ed. Seoul: Korean Society of Plant Pathology; 2009.
3 White TJ, Bruns TD, Lee SB, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, editors. PCR protocols: a guide to methods and applications. San Diego: Academic Press; 1990. p. 315-22.
4 Mordue JE. Sclerotinia rolfsii. In: Commonwealth Mycological Institute (Great Britain), CAB International, editors. CMI descriptions of pathogenic fungi and bacteria. Wallingford: CAB International;1974. no. 410.
5 Kwon JH, Lee HS, Kim J, Kim WI, Shim HS, Shen SS. Sclerotium rot of Cyclamen europaeum caused by Sclerotium rolfsii. Res Plant Dis 2014;20:223-6.   DOI
6 Kwon JH, Hwang GC, Lee SB, Choi YJ, Lee SD, Nguyen LM. Sclerotium rot of Pulsatilla koreana Nakai caused by Sclerotium rolfsii. Res Plant Dis 2015;21:334-6.   DOI
7 Adegbite AA, Amusa NA. The major economic field diseases of cowpea in the humid agro-ecologies of South-Western Nigeria. Afr J Biotechnol 2008;7:4706-12.