Browse > Article
http://dx.doi.org/10.4489/KJM.2015.43.1.20

Diversity of Endophytic Fungi Isolated from Roots of Cypripedium japonicum and C. macranthum in Korea  

Lee, Bong-Hyung (Department of Biology Education, Korea National University of Education)
Han, Han-Kyeol (Department of Biology Education, Korea National University of Education)
Kwon, Hye-Jin (Plant Conservation Division, Korea National Arboretum)
Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
Publication Information
The Korean Journal of Mycology / v.43, no.1, 2015 , pp. 20-25 More about this Journal
Abstract
This study was conducted to investigate diversity of orchid endophytic fungi from roots of two terrestrial orchids, Cypripedium japonicum and Cypripedium macranthum in Korea. The endophytic fungi were identified using morphological and DNA sequences isolated from the roots. Totally, 11 taxa of the endophytic fungi were identified from the roots of C. japonicum and 15 taxa from C. macranthum. Three species of the fungi were common in both host species; Leptodontidium orchidicola, Humicola fuscoatra var. fuscoatra, Umbelopsis dimorpha. Six species of the fungal isolates were the first report in Korea; Oidiodendron echinulatum, Pseudogymnoascus roseus, Geomyces vinaceus, Cryptosporiopsis ericae, U. dimorpha, Chaetomium cupreum.
Keywords
Cypripedium japonicum; Cypripedium macranthum; Fungal Endophytes; Orchid Roots;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Zelmer CD. Interactions between northern terrestrial orchids and fungi in nature [dissertation]. Edmonton (AB): University of Alberta; 1994.
2 Simard SW, Perry DA, Jones MD, Myrold DD, Durall DM, Molina R. Net transfer of carbon between ectomycorrhizal tree species in the field. Nature 1997;388:579-82.   DOI   ScienceOn
3 Rasmussen HN. Recent developments in the study of orchid mycorrhiza. Plant Soil 2002;244:149-63.   DOI   ScienceOn
4 Currah RS, Sigler L, Hambleton S. New records and new taxa of fungi from the mycorrhizae of terrestrial orchids of Alberta. Can J Bot 1987;65:2473-82.   DOI
5 Fernando AA, Currah RS. Leptodontidium orchidicola (Mycelium radicis atrovirens complex): aspects of its conidiogenesis and ecology. Mycotaxon 1995;54:287-94.
6 Gorzelak MA, Hambleton S, Massicotte HB. Community structure of ericoid mycorrhizas and root-associated fungi of Vaccinium membranaceum across an elevation gradient in the Canadian Rocky Mountains. Fungal Ecol 2012;5:36-45.   DOI   ScienceOn
7 Rasmussen HN. Terrestrial orchids: from seed to mycotrophic plant. New York: Cambridge University Press; 1995.
8 Leake JR. The biology of myco-heterotrophic ('saprophytic') plants. New Phytol 1994;127:171-216.   DOI   ScienceOn
9 Carroll G. Fungal endophytes in stems and leaves: from latent pathogen to mutualistic symbiont. Ecology 1988;69:2-9.   DOI   ScienceOn
10 Currah RS, Zelmer CD, Hambleton S, Richardson KA. Fungi from orchid mycorrhizas. In: Arditti J, Pridgeon A, editors. Orchid biology: reviews and perspectives, VII. Dordrecht: Kluwer Academic Publishers; 1997. p. 117-70.
11 Bernard N. L'evolution dans la symbiose: les orchidees et leurs champignons commensaux. Paris: Masson et Cie, Editeurs; 1909.
12 Vujanovic V, St-Arnaud M, Barabe D, Thibeault G. Viability testing of orchid seed and the promotion of colouration and germination. Ann Bot 2000;86:79-86.   DOI   ScienceOn
13 Arnold AE, Maynard Z, Gilbert GS. Fungal endophytes in dicotyledonous neotropical trees: patterns of abundance and diversity. Mycol Res 2001;105:1502-7.   DOI   ScienceOn
14 Lee BC, Yang HH, Kim JS. Rare plants data book in Korea. Pocheon: Korea National Arboretum; 2009.
15 Sim MY, Youm J, Chung J, Lee B, Koo CD, Eom AH. Characteristic of orchid mycorrhizal fungi from roots of Cypripedium japonicum and C. macranthum. Kor J Mycol 2010;38:1-4.   DOI   ScienceOn
16 Richardson KA, Currah RS, Hambleton S. Basidiomycetous endophytes from the roots of neotropical epiphytic Orchidaceae. Lindleyana 1993;8:127-37.
17 Gardes M, Bruns T. ITS primers with enhanced specificity for basidiomycetes: application to the identification of mycorrhizae and rusts. Mol Ecol 1993;2:113-8.   DOI   ScienceOn
18 Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731-9.   DOI   ScienceOn