Browse > Article
http://dx.doi.org/10.4489/KJM.2014.42.2.165

Germination and Antioxidant Activity of Korean Oak Pollen Treated with Medicinal Mushrooms  

Hong, In-Pyo (National Academy of Agricultural Science and Technology, Rural Development Administration)
Woo, Soon-Ok (National Academy of Agricultural Science and Technology, Rural Development Administration)
Han, Sang-Mi (National Academy of Agricultural Science and Technology, Rural Development Administration)
Yeo, Joo-Hong (National Academy of Agricultural Science and Technology, Rural Development Administration)
Cho, Mi-Lan (National Academy of Agricultural Science and Technology, Rural Development Administration)
Publication Information
The Korean Journal of Mycology / v.42, no.2, 2014 , pp. 165-169 More about this Journal
Abstract
In this study, we measured antioxidant activity as DPPH radical scavenging and the total polyphenol content of pulverized and lyophilized oak pollens inoculated with fungi to confirm the husk removal effect. The total polyphenol content of oak pollen was highest in lyophilized pollen medium inoculated with Armillaria mellea, and was lowest in pollen inoculated with Lentinula edodes. Total polyphenol content of the lyophilized pollen was higher than that of the refined pollen and the pulverized pollen in oak pollen germinated with A. mellea. The total polyphenol content of the lyophilized oak pollen germinated with A. mellea was 1.4-fold higher than that extracted with water. Measurement of antioxidant activity using the DPPH (2, 2 diphenyl-1-picrylhydrazyl) free radical scavenging method showed that the lyophilized oak pollen germinated with A. mellea had the highest and that germinated with L. edodes was lowest in antioxidant activities. The lyophilized oak pollen germinated with A. mellea was 2 to 4 times higher than that extracted with water in the antioxidant activity of DPPH free radical scavenging. Many germinated cells were formed around pore of acorn pollen inoculated with L. edodes, while those were formed at the end of hyphae derived from oak pollen inoculated with A. mellea.
Keywords
Antioxidant activity; Armillaria mellea; Bee pollen; Lyophilization;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Han MR, Lee SJ, Kim MH. Development of pine pollen cell wall rupture technique using a high impact planetary milling process. Dankook J New Material Technol 2004;12:43-54.
2 Schwarze FWMR, Engels J, Mattheck C. Fungal Strategies of Wood Decay in Trees. Springer; 2000. p. 61-4.
3 Kress WJ, Stone DE, Sellers SC. Ultrastructure of exine-less pollen: Heliconia (Heliconiaceae). Amer J Bot 1978;65:1064-76.   DOI   ScienceOn
4 Andrade P, Ferreres F, Gil MI, Tomas-Barberan FA. Determination of phenolic compounds in honeys with different floral origin by capillary zone electrophoresis. Food Chemistry 1997;60:79-84.   DOI   ScienceOn
5 Choi SJ, Jeong YH. Effect of proteases on the extraction of crude protein and reducing sugar in pollen. J Kor Soc Food Sci Nutr 2004;33:1353-8.   과학기술학회마을   DOI
6 Fang KF, Wang YN, Yu TQ, Zhang LY, Baluska F, Samaj J, Lin JX. Isolation of de-exined pollen and cytological studies of the pollen intines of Pinus bungeana Zucc. Ex Endl. and Picea wilsonii Mast. Flora 2008;203:332-40.
7 Blois MS. Antioxidants determination by the use of a stable free radical. Nature 1958;181:1199-200.   DOI   ScienceOn
8 Liangli Y, Scott A, Jonathan P, Mary H, John W, Ming Q. Antioxidant properties of hard winter wheat extracts. J Agric Food Chem 2002;60:1619-24.
9 Chung YG, Yoon SH, Kwon JS, Bae MJ. Nutritional and biochemical studies on the pollen loads studies on lipid compositions of sunflower pollen load and effects of its pollen load on liver cholesterol metabolism in mouse. J Kor Soc Food Nutr 1984;13:169-74.
10 Li F, Yuan QP, Rashid F. Isolation, purification and immunobiological activity of a new water-soluble bee pollen polysaccharide from Crataegus pinnatifida Bge. Carbohydr Polym 2009;78:80-8.   DOI   ScienceOn
11 Block G, Langseth L. Antioxidant vitamins and disease prevention. Food Technol 1994;48:80-4.