Browse > Article
http://dx.doi.org/10.4489/KJM.2014.42.1.1

Genetic Diversity of Agaricus bisporus Strains by PCR Polymorphism  

Min, Kyong-Jin (Graduate School of Future Convergence Technology, Hankyong National University)
Kim, Jong-Kun (JK BioTech. Co. Ltd.)
Kwak, A-Min (Graduate School of Future Convergence Technology, Hankyong National University)
Kong, Won-Sik (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA)
Oh, Youn-Hee (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA)
Kang, Hee-Wan (Graduate School of Future Convergence Technology, Hankyong National University)
Publication Information
The Korean Journal of Mycology / v.42, no.1, 2014 , pp. 1-8 More about this Journal
Abstract
Twelve Universal fungal PCR fingerprint (UFPF) primers that were modified from Universal rice primer (URP) were used to assess genetic diversity of 64 Agaricus strains including 45 A. bisporus strains and other 19 strains of other Agaricus spp. Eight primers, UFPF1, UFPF2, UFPF3, UFPF7, UFPF9, UFPF10, UFPF11, and UFPF12 produced PCR polymorphic bands within and between the Agaricus species. Primer UFPF7 produced specific PCR polymorphic bands that are distinct Korean strain from different strains. Ninety five PCR polymorphic bands were inputted for UPGMA cluster analysis. Forty five strains of A. bisporus are genetically clustered into 8 groups, showing coefficient similarity from 0.75 to 0.9 among them. The varieties, Saea, Saedo, Saejeong and Saeyeon that have recently been developed in Korea were involved in the same group with close genetic relationship of coefficient similarity over 0.96, whereas, other Korean strains were genetically related to A. bisporus strains that were introduced from USA, Eroupe and Chinese.
Keywords
Agaricus bisporus strains; Genetic diversity; PCR polymorphism;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Loftus M, Bouchti-King L, Robles C. Use of SCAR marker for cap color in Agaricus bisporus breeding programs. Mush Sci 2000;5:201-205.
2 Malekzadeh K, Banafsheh JMS, Ehsan M. Use of ISSR markers for strain identification in the button mushroom, Agaricus bisporus. In: Savoie JM, Foulongne-Oriol M, Largeteau M, Barroso G, editors, Proceedings of the 7th International Conference on Mushroom Biology and Mushroom Products, Arcachon, France; 2011. p. 3-34
3 Sonnenberg ASM, Johan JPB, Patrick MH, Brian L, Wei G, Amrah W, Jurriaan JM. Breeding and strain protection in the button mushroom Agaricus bisporus. In: Savoie JM, Foulongne- Oriol M, Largeteau M., Barroso G, editors, Proceedings of the 7th International Conference on Mushroom Biology and Mushroom Products, Arcachon, France; 2011. p. 7-15.
4 Fritsche G. Breeding Agaricus bisporus at the mushroom experimental station. Horst Mushroom J 1983;157:4-17
5 Foulongne-Oriol M., Spataro C, Savoie JM. Microsatellite markers isolated in the white button mushroom Agaricus bisporus are suitable for genetic studies. Appl Microbiol Biotechnol 2009;84:1125-1135   DOI
6 Khush RS, Becker E, Wach M. DNA amplification polymorphisms of the cultivated mushroom Agaricus bisporus. Appl Environ Microbiol 1992;58:2971-2977.
7 Yadav MC, Mahfooz S, Singh SK, Upadhyay RC. RAPD markers assisted selection of genetically diverse parents for hybridization in Agaricus bisporus. Mushroom Res 2003;12: 19-26.
8 Yadav MC, Challen MP, Singh SK, Elliott TJ. DNA analysis reveals genomic homogeneity and single nucleotide polymorphism in 5.8S ribosomal RNA gene spacer region among commercial cultivars of the button mushroom Agaricus bisporus in India. Curr Science 2007;93:1383-1391.
9 Kang HW, Park DS, Go SJ, Eun MY. Fingerprinting of diverse genomes using PCR with universal rice primers generated from repetitive sequence of Korean weedy rice. Mol Cells 2002;13:281-287.
10 Kang HW. Genetic divesity analysis of fungal species by universal rice primer (URP)-PCR. Kor J Mycol. 2012;40:78-85.   과학기술학회마을   DOI
11 Kang HW, Park DS, Park YJ, You CH, Lee BM, Go SJ. Genomic differentiation among oyster mushroom cultivars released in Korea by URP-PCR fingerprinting. Mycobiology 2001; 29:85-89.   과학기술학회마을
12 Kim HK, Ham IG, Lee KS, Lee BJ, Kim YG, Yang ES, Yoo YB, Kim HG. Characteristics of a new button mushroom variety Dahyang. J Mushroom Sci Prod 2011;9:17-21.
13 Oh YL, Jang KY, Jhune CS, Kong WS, Yoo YB, Shin PG, Seo JS. Quality changes in Agaricus bisporus varieties due to period and temperature during their storage. J Mushroom Sci Prod 2013;11:137-144   과학기술학회마을   DOI
14 Foulongne-Oriol M, Spataro C, Cathalot V, Monllor S, Savoie JM. An expanded genetic linkage map of an intervariental Agaricus bisporus var. bisporus x A. bisporus var. burnettii hybrid based on AFLP, SSR and CAPS markers sheds light on the recombination behaviour of the species. Fung Genet Biol 2010;47:226-236.   DOI   ScienceOn
15 Wang ZS, Chen MY, Cai ZX, Liao JH, Li HR, Guo ZJ, Lu ZH. DNA fingerprinting of genetic diversity of Agaricus bisporus. In: Savoie JM, Foulongne-Oriol M, Largeteau M., Barroso G, editors, Proceedings of the 7th International Conference on Mushroom Biology and Mushroom Products, Arcachon, France; 2011. p. 1-8.
16 Kang HW, Park DS, Park YJ, Lee BM, Cho SM, Kim KT, Seo KS, Go SJ. PCR based detection of Phellinus linteus using specific primers Generated from Universal Rice Primer (URP) derived PCR polymorphic band. Mycobiology 2002;30:202-207.   과학기술학회마을   DOI