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http://dx.doi.org/10.4489/KJM.2013.41.4.287

Genetic Transformation of the Mycelia of Tremella fuciformis and Changes of Cytotoxicity  

Shin, Dong-Il (Department of Biotechnology, Catholic University of Daegu)
Park, Hee-Sung (Department of Biotechnology, Catholic University of Daegu)
Publication Information
The Korean Journal of Mycology / v.41, no.4, 2013 , pp. 287-291 More about this Journal
Abstract
Tremella fuciformis, as one of higher basidiomycetes, can asexually reproduce yeast-like conidium (YLC) cells by budding. We have developed an efficient method to introduce pCambia1300 plasmid containing hph gene into YLC cells using Agrobacterium. This was successful only when YLC cells were wounded by NaOH treatment before co-cultivation. In average, 40~50 transformants were produced out of $1.0{\times}10^6$ YLC cells investigated. The T-DNA transfer was confirmed by PCR. Methanolic extracts from transformants demonstrated different levels of toxicity against SKOV-3 cervical cancer cells.
Keywords
Agrobacterium method; Conidia; Cytotoxicity; Tremella fuciformis;
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