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http://dx.doi.org/10.4489/KJM.2013.41.4.268

Biological Control of Cucumber Powdery Mildew by Bacillus amyloliquefaciens M27  

Lee, Sang Yeob (Agricultural Microbiology Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA))
Weon, Hang Yeon (Agricultural Microbiology Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA))
Kim, Jeong Jun (Agricultural Microbiology Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA))
Han, Ji Hee (Agricultural Microbiology Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA))
Kim, Wan Gyu (Agricultural Microbiology Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA))
Publication Information
The Korean Journal of Mycology / v.41, no.4, 2013 , pp. 268-273 More about this Journal
Abstract
Bacillus amyloliquefaciens M27 was isolated from the cotton-waste compost for cultivation of oyster mushroom (Pleurotus ostreatus). B. amyloliquefaciens M27 is a biocontrol agent with antagonistic activities against a wide range of fungal pathogens. The aim of this work was to evaluate the possibility of exploiting antagonistic bacteria, B. amyloliquefaciens M27, in the biological control of the cucumber powdery mildew fungus, Podosphaera fusca. In greenhouse tests, the isolate was found to be very effective to control powdery mildew on cucumber leaves showing 4.0% diseased area, whereas diseased area in the control was 80.5%. The filtrate of the isolate cultured on MH and LB media were more effective for control of the disease than those cultured on TSB, NB, and KB media. When two, five, ten, 20, 50 and 100-fold diluted culture broth of isolate on LB media were treated, disease areas were 0%, 0%, 0%, 1.3%, 3.1% and 5.0%, respectively, whereas diseased area in the control was 60.0%. The filtrate of the isolate cultured on LB media was treated to cucumber plants on July, October and December just before the outbreak of the powdery mildew occurred. When 10-fold diluted filtrate of the isolate was treated, control efficacy was 88.9~98.9% in the treated seasons. The results showed that the culture filtrate of B. amyloliquefaciens M27 was very effective to control powdery mildew of cucumber.
Keywords
Bacillus amyloliquefaciens; Biological control; Cucumber; Powdery mildew;
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