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http://dx.doi.org/10.4489/KJM.2013.41.4.248

Isolation of the Bacterium Pseudomonas sp. HC1 Effective in Inactivation of Tolaasin Produced by Pseudomonas tolaasii  

Lee, Chan-Jung (Mushroom Research Division, NIHHS, RDA)
Yoo, Young-Mi (Mushroom Research Division, NIHHS, RDA)
Han, Ju-Yeon (Mushroom Research Division, NIHHS, RDA)
Jhune, Chang-Sung (Mushroom Research Division, NIHHS, RDA)
Cheong, Jong-Chun (Mushroom Research Division, NIHHS, RDA)
Moon, Ji-Won (Mushroom Research Division, NIHHS, RDA)
Suh, Jang-Sun (Mushroom Research Division, NIHHS, RDA)
Han, Hye-Su (Heuksalim)
Cha, Jae-Soon (Department of Plant Medicine, Chungbuk National University)
Publication Information
The Korean Journal of Mycology / v.41, no.4, 2013 , pp. 248-254 More about this Journal
Abstract
A Gram-negative bacterium was isolated from mushroom media that markedly reduces the level of extracellular toxins (i.e., tolaasins) produced by Pseudomonas tolaasii, the most destructive pathogen of cultivated mushrooms. The HC1 strain was selected as detoxifying tolaasin by bioassay on potato and it was identified Pseudomonas sp. by the cultural, morphological and physiological characteristics, and analysis of the 16S rRNA. The isolated bacterium is saprophytic but not parasitic nor pathogenic to cultivation mushroom. The isolated bacterium for P. tolaasii cell, was sufficient for detoxification in vitro. Inoculation of the isolated bacterium prevents the development of bacterial disease in Pleurotus ostreatus, Flammunia velutipes and Agaricus bisporus. Control efficacy of brown blotch of strain HC1 treatment was 69, 68 and 55% on Agaricus bisporus, Flammulina velutipes and Pleurotus ostreatus, respectively. The suppressive bacterium may be useful in future for the development of biocontrol system and the construction of genetically modified edible fungi resistant to the disease caused by P. tolaasii.
Keywords
Control efficacy; Detoxify; Mushrooms; Pseudomonas tolaasii; Tolaasin;
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