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http://dx.doi.org/10.4489/KJM.2012.40.4.288

Investigation of Fungi in Pesticide Fumigated Oak Wilt-Diseased Logs  

Suh, Dong Yeon (Department of Microbiology and Institute of Basic Sciences, Dankook University)
Son, Seong-Yeol (Department of Microbiology and Institute of Basic Sciences, Dankook University)
Kim, Seong Hwan (Department of Microbiology and Institute of Basic Sciences, Dankook University)
Seo, Sang Tae (Division of Forest Insect Pests and Diseases, Korea Forest Research Institute, Korea Forest Service)
Kim, Kyung Hee (Division of Forest Insect Pests and Diseases, Korea Forest Research Institute, Korea Forest Service)
Ko, Han Kyu (Forest Mushroom Research Center, National Forestry Cooperative Federation)
Publication Information
The Korean Journal of Mycology / v.40, no.4, 2012 , pp. 288-291 More about this Journal
Abstract
Korean oak wilt disease caused by Raffaelea quercus-mongolicae is vectored by the ambrosia beetle Platypus koryoensis. To prevent the spread of the disease, the beetle infested oak tree had been cut into logs, covered with plastic vinyl, fumigated with a pesticide, and stored for three years on the site where the tree was cut. This study was carried out to get information on the fungi colonizing the fumigated oak wood. Wood disk samples collected from the fumigated oak logs at two locations in the Taejo Mountain, Cheonan city, were used for fungal isolation. A total of 99 filamentous fungal isolates were obtained from the wood disk samples. Hypocrea spp., Trichoderma spp. and Penicillium spp. were identified based on morphological characteristics and nucleotide sequence analysis of translation elongation factor 1-alpha gene and ITS rDNA region. Trichoderma was the major fungal group. R. quercus-mongolicae, and P. koryoensis were not detected from the fumigated oak wood. Our work provided evidence that after three years of storage, the fumigated oak wilt-diseased logs should be no longer harmful source of oak wilt disease transmission.
Keywords
Fungal flora; Fumigation; Oak wilt disease; Platypus koryoensis; Raffaelea quercus-mongolicae;
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1 Evidente, A., Ricciardiello, G., Andolfi, A., Sabatini, M. A., Ganass, I. S., Altomare, C., Favilla, M. and Melck, D. 2008. Citrantifidiene and citrantifidiol: bioactive metabolites produced by Trichoderma citrinoviride with potential antifeedant activity toward aphids. J. Agric. Food Chem. 56:3569-3573.   DOI   ScienceOn
2 Kim, J. Y., Yun, Y. H., Hyun, M. W., Kim, M. H. and Kim, S. H. 2010. Identification and characterization of Gliocladium viride isolated from mushroom fly infested oak log used for shiitake cultivation. Mycobiology 38:7-12.   DOI   ScienceOn
3 Kim, J. Y., Kwon, H. W., Tang, L. and Kim, S. H. 2012. Identification and characterization of Trichoderma citrinoviride isolated from mushroom fly-infested oak log beds used for shiitake cultivation. Plant Pathol. J. 28:219.   DOI   ScienceOn
4 Kim, K. H., Choi, Y. J., Seo, S. T. and Shin, H. D. 2009. Raffaelea quercus-mongolicae sp. nov. associated with Platypus koryoensis on oak in Korea. Mycotaxon 110:189-197.   DOI
5 Mandels, M. and Reese, E. T. 1957. Induction of cellulase in Trichoderma viride as influenced by carbon sources and metals. J. Bacteriol. 73:269-278.
6 Suh, D. Y., Hyun, M. W., Kim, S. H., Seo, S. T. and Kim, K. H. 2011. Filamentous fungi isolated from Platypus koryoensis, the insect vector of oak wilt disease in Korea. Mycobiology 39:314- 316.
7 Overton, B. E., Stewart, E. L. and Geiser, D. M. 2006. Taxonomy and phylogenetic relationships of nine species of Hypocrea with anamorphs assignable to Trichoderma section Hypocreanum. Stud. Mycol. 56:39-65.   DOI   ScienceOn
8 Richard, A. H., Nichole, B. and Stephen, J. V. 2002. Evaluation of rapid DNA extraction methods for the quantitative detection of fungi using real-time PCR analysis. J. Microbiol. Methods 50:319-323.   DOI   ScienceOn
9 Samuels, G. J., Dodd, S. L., Lu, B.-S., Petrini, O., Schroers, H.-J. and Druzhinina, I. S. 2006. The Trichoderma koningii aggregate species. Stud. Mycol. 56: 67-133.   DOI   ScienceOn
10 White, T. J., Bruns, T., Lee, S. and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: A guide to methods and applications, pp. 315-322. Academic Press, Sandiego.