Browse > Article
http://dx.doi.org/10.4489/KJM.2010.39.3.243

Bioethanol Production using a Yeast Pichia stipitis from the Hydrolysate of Ulva pertusa Kjellman  

Lee, Ji-Eun (Department of Biotechnology, Chungju National University)
Lee, Sang-Eun (Department of Biotechnology, Chungju National University)
Choi, Woon-Yong (Division of Biomaterials Engineering, Kangwon National University)
Kang, Do-Hyung (Korea Ocean Research & Development Institute)
Lee, Hyeon-Yong (Division of Biomaterials Engineering, Kangwon National University)
Jung, Kyung-Hwan (Department of Biotechnology, Chungju National University)
Publication Information
The Korean Journal of Mycology / v.39, no.3, 2011 , pp. 243-248 More about this Journal
Abstract
We studied the repeated-batch process for the bioethanol production from the hydrolysate of Ulva pertusa Kjellman using yeast Pichia stipitis, which is able to assimilate C6- and C5-monosaccharides. During 180-hour operations, the repeated-batch process was carried out stably, and the average bioethanol concentration reached 11.9 g/L from about 30 g/L of reducing sugar in the hydrolysate. Meanwhile, the bioethanol yields, based on the reducing sugar and the quantitative TLC analysis, were 0.40 and 0.37, respectively, which corresponded to 78.4% and 72.5% of theoretical value, respectively. Throughout the quantitative process analysis, it was also demonstrated that 39.67 g-bioethanol could be produced from 1 kg of dried Ulva pertusa Kjellman. In this study, we verified that the bioethanol production from the hydrolysate of Ulva pertusa Kjellman was feasible using a yeast Pichia stipitis, particularly during the repeated-batch operation.
Keywords
Bioethanol; Pichia stipitis; Repeated-batch; Ulva pertusa Kjellman;
Citations & Related Records
Times Cited By KSCI : 7  (Citation Analysis)
연도 인용수 순위
1 이성목, 김재혁, 조화영, 주현, 이재화. 2009a. 물리화학적 가수분해에 의한 갈조류 바이오 에탄올 생산. 공업화학. 20:517-521.   과학기술학회마을
2 이성목, 최인순, 김성구, 이재화. 2009b. 효소적 가수분해에 의한 갈조류 바이오 에탄올 생산. 한국생물공학회지. 24:483-488.   과학기술학회마을
3 한재건, 오성호, 최운용, 권정웅, 서현범, 정경환, 강도형, 이현용. 2010. 고압액화공정을 이용한 구멍갈파래의 발효용 알코올 당화수율 증진. 한국생물공학회지. 25:357-362.   과학기술학회마을
4 Yeon, J.-H., Lee, S.-E., Choi, W. Y., Kang, D. H., Lee, H.-Y and Jung, K.-H. 2011a. Repeated-batch operation of surfaceaerated fermentor for bioethanol production from the hydrolysate of seaweed Sargassum sagamianum. J. Microbiol. Biotechnol. 21:323-331.
5 Yeon, J.-H., Lee, S.-E., Choi, W. Y., Choi, W.-S., Kim, I.-C., Lee, H.-Y. and Jung, K.-H. 2011b. Bioethanol production from the hydrolysate of rape stem in a surface-aerated fermentor. J. Microbiol. Biotechnol. 21:109-114.   DOI   ScienceOn
6 Qi, H., Zhang, Q., Zhao, T., Hu, R., Zhang, K. and Li, Z. 2006. In vitro antioxidant activity of acetylated and benzoylated derivatives of polysaccharide extracted from Ulva pertusa (Chlorophyta). Bioorg. Med. Chem. Lett. 16:2441-2445.   DOI   ScienceOn
7 Pengzhan, Y., Ning, L., Xiguang, L., Gefei, Z., Quanbin, Z. and Pengcheng, L. 2003a. Antihyperlipidemic effects of different molecular weight sulfated polysaccharides from Ulva pertusa (Chlorophyta). Pharmacol. Res. 48:543-549.   DOI   ScienceOn
8 Pengzhan, Y., Quanbin, Z., Ning, L., Zuhong, X. Yanmei, W. and Li Zhi'en, L. 2003b. Polysaccharides from Ulva pertusa (Chlorophyta) and preliminary studies on their antihyperlipidemia activity. J. Appl. Phycol. 15:121-127.   DOI   ScienceOn
9 Qi, H., Zhang, Q., Zhao, T., Chen, R., Zhang, H., Niu, X. and Li, Z. 2005a. Antioxidant activity of different sulfate content derivatives of polysaccharide extracted from Ulva pertusa (Chlorophyta) in vitro. Int. J. Biol. Macromol. 37:4195-199.
10 Qi, H., Zhao, T., Zhang, Q., Li, Z., Zhao Z. and Xing, R. 2005b. Antioxidant activity of different molecular weight sulfated polysaccharides from Ulva pertusa Kjellm (Chlorophyta). J. Appl. Phycol. 17:527-534.   DOI
11 Robyt, J. F. and Mukerjea, R. 1994. Separation and quantitative determination of nanogram quantities of maltodextrins and isomaltodextrins by thin-layer chromatography. Carbohydr. Res. 251:187-202.   DOI   ScienceOn
12 Skoog, K., and Hahn-Hagerdal, B. 1990. Effect of oxygenation on xylose fermentation by Pichia stipitis. Appl. Environ. Microbiol. 56:3389-3394.
13 Akakabe, Y., Matsui, K. and Kajiwara, T. 1999. Enantioselective ${\alpha}-hydroperoxylation$ of long-chain fatty acids with crude enzyme of marine green alga Ulva pertusa. Tetrahedron Lett. 40:1137-1140.   DOI   ScienceOn
14 Watson, N. E., Prior, B. A., du Preez, J. C. and Lategan, P. M. 1984. Oxygen requirements for D-xylose fermentation to ethanol and polyols by Pachysolen tannophilus. Enzyme Microb. Technol. 6:447-450.   DOI   ScienceOn
15 한재건, 하지혜, 이현용, 최영범, 고정임, 강도형. 2009. 구멍갈파래의 면역활성 증진을 위한 추출방법 비교. 한국식품과학회지. 41:380-385.   과학기술학회마을
16 Adams, J. M., Gallagher, J. A. and Donnison, I. S. 2009. Fermentation study on Saccharina latissima for bioethanol production considering variable pre-treatments. J. Appl. Phycol. 21:569-574.   DOI
17 Chaplin, M. F. and Kennedy, J. F. 1986. Carbohydrate analysis; A practical approach, pp. 3. IRL Press, Oxford, UK.
18 Grootjen, D. R. J., van der Lans, R. G. J. M. and Luyben, K. Ch. A. M. 1990. Effects of the aeration rate on the fermentation of glucose and xylose by Pichia stipitis CBS 5773. Enzyme Microb. Technol. 12:20-23.   DOI   ScienceOn
19 Harun, R., Danquah, M. K. and Forde, G. M. 2010. Microalgal biomass as a fermentation feedstock for bioethanol production. J. Chem. Technol. Biotechnol. 85:199-203.
20 Horn, S. J., Aasen, I. M. and Ostgaard, K. 2000. Ethanol production from seaweed extract. J. Ind. Microbiol. Biotechnol. 25:249-254.   DOI
21 Hull, S. R. Yang, B. Y. Venzke, D., Kulhavy, K. and Montgomery, R. 1996. Composition of corn steep water during steeping J. Agric. Food Chem. 44:1857-1863.   DOI   ScienceOn
22 연지현, 서현범, 오성호, 최원석, 강도형, 이현용, 정경환. 2010. 모자반 가수분해물을 이용한 바이오 에탄올 생산. 한국생물공학회지. 25:283-288.   과학기술학회마을
23 Jeffries, T. W. 2006. Engineering yeasts for xylose metabolism. Curr. Opin. Biotechnol. 17:320-326.   DOI   ScienceOn
24 Ligthelm, M. E., Prior, B. A. and du Preez, J. C. 1988. The oxygen requirements of yeasts for the fermentation of D-xylose and D-glucose to ethanol. Appl. Microbiol. Biotechnol. 28:63-68.   DOI
25 배태진, 강동수, 최옥수. 2000. 구멍갈파래(Ulva pertusa)로부터 $Dimethyl{\beta}propiothetin$ 최적추출조건. 한국식품영양과학회지. 29:783-789.   과학기술학회마을