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http://dx.doi.org/10.4489/KJM.2010.39.3.180

Genetic Diversity of Paecilomyces japonica and Cordypces militaris Strains by URP-PCR Fingerprinting  

Kim, Jong-Kun (JK BioTech Co Ltd.)
Kang, Hee-Wan (Graduate School of Biotechnology and Information Technology, Hankyong National University)
Publication Information
The Korean Journal of Mycology / v.39, no.3, 2011 , pp. 180-184 More about this Journal
Abstract
This study was carried out to identify the genetic characteristics among isolates of Paecilomyces spp.and Cordyceps spp. by URP-PCR analysis. Twenty URP (universal rice primer) primers of 20 mer which were designed from repetitive sequence of rice, were used for producing PCR DNA fingerprints of the mushrooms. Of them, 5 URP primers, URP2F, URP2R, URP9F, URP4R, and URP17R amplified genomic DNA of the mushrooms with polymorphic PCR patterns. On isolates of Cordyceps militaris, primers URP1F, URP2R, URP6R and URP17R produced PCR polymorphic bands of 4 types. Isolates of Cordypces sp. that are isolated from different area of Korea were identical to isolate of C. militaris, while other species of Cordypces were different to the PCR profiles. However, the URP primers did not identify the polymorphism of PCR profile on isolates of P. japonica.
Keywords
Cordyceps spp.; Paecilomyces spp.; Genetic diversity; URP-PCR;
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