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http://dx.doi.org/10.4489/KJM.2010.38.2.167

Inhibitory Effects of Melanin Secretion on B16 Melanoma cell of Cordyceps militaris Water Extract  

Nam, Byung-Hyouk (Department of Microbiology, College of Natural Sciences, Pusan National University)
Jo, Wool-Soon (Dong Nam Institute of Radiological & Medical Sciences)
Choi, Yoo-Jin (Dong Nam Institute of Radiological & Medical Sciences)
Lee, Jae-Yun (Department of Microbiology, College of Natural Sciences, Pusan National University)
Kang, Eun-Young (Busan Techno-park Marine Bio-Industry Development Center)
Jeong, Min-Ho (Department of Microbiology, Dong-A University Medical College)
Lee, Jae-Dong (Department of Microbiology, College of Natural Sciences, Pusan National University)
Publication Information
The Korean Journal of Mycology / v.38, no.2, 2010 , pp. 167-171 More about this Journal
Abstract
The present study aims to evaluate Cordyceps militaris water extract (CMWE) with a view to develop melanogenesis inhibitors. Inhibitory activities of CMWE against tyrosinase, L-DOPA(L-3,4-dihydroxyphenylalanine) oxidation, and melanin biosynthesis in B16 mouse melanoma cells were investigated. CMWE, at $5000\;{\mu}g/ml$, inhibited tyrosinase activity of 71% and DOPA oxidation of 40% as reacting with L-DOPA. Furthermore, B16 mouse melanoma cell survived over 50% from low to high dose on MTT assay, and CMWE markedly inhibited (> 50%) melanin synthesis at $5000\;{\mu}g/ml$. The inhibitory effect of CMWE on melanogenesis was attributed to enhancement of tyrosinase degradation. Key enzyme of melanin biosynthesis is tyrosinase which catalyses a beginning step from tyrosine to DOPA quinine and melanin formation step, respectively. These results indicated that CMWE may be a potential source of novel whitening agents for cosmetic or therapeutic application.
Keywords
Melanogenesis; Melanoma; Militaris; Tyrlsinase; Whitening;
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Times Cited By KSCI : 11  (Citation Analysis)
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