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http://dx.doi.org/10.4489/KJM.2010.38.1.069

Effect of Hydrophobicity of Oyster Mushroom on the Severity of Brown Blotch  

Lee, Ki-Young (Department of Agricultural Biology, Chungbuk National University)
Yang, Seung-Up (Department of Agricultural Biology, Chungbuk National University)
Han, Jong-Woo (Department of Agricultural Biology, Chungbuk National University)
Yoon, Kun-Sik (Department of Agricultural Biology, Chungbuk National University)
Jang, Hoo-Bong (Chungbuk Agricultural Research & Extension services)
Cha, Jae-Soon (Department of Agricultural Biology, Chungbuk National University)
Publication Information
The Korean Journal of Mycology / v.38, no.1, 2010 , pp. 69-74 More about this Journal
Abstract
The 2 strains of Pleurotus ostreatus showing very severe and mild symptom of brown blotch were selected, and their F1 hybrid strains were made by mating of their single spore isolates. Hydrophobicity of fruiting body surface and brown blotch severity of the parental and their 11 F1 hybrid strains showing different level of severity of brown blotch were determined. Correlation coefficient (R) between disease severity and hydrophobicity were 0.68 and 0.70 on two independent experiments. Correlation coefficient between disease severity and disease incidence that was determined in the oyster mushroom farm were 0.46 and 0.52 on two independent experiments. When GFP-tagged Pseudomonas tolaasii was monitored with confocal microscope on cap surface of fruiting body, more cells of the pathogen were clustered on the severe strain than the mild strain, which indicates that the bacterial pathogen proliferates more on the severe strain. These results suggest that hydrophobicity of oyster mushroom fruiting body affects disease severity of the brown blotch disease; the longer the bacterial suspension stays on fruiting body surface more severe symptom of the blotch develops.
Keywords
Bacterial brown blotch; Confocal microscope; Disease severity; Hydrophobicity; Pseudomonas tolaasii;
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1 Robert, C., Levente, B., Jozsef, K., Colin M. J., and Kornel L. K. 2003. Genes involved in the copper-dependent regulation of soluble methane monooxygenase of Methyloccus capsulatus (Bath): cloning, sequencing and mutational analysis. Microbiology 149:1785-1795.   DOI   ScienceOn
2 Shirata A., Sugaya, K., Takasugi, M., and Monde, K. 1995. Isolation and biological activity of toxins produced by a Japanese strain of Pseudomonas tolaasii the pathogen of bacterial rot of cultivated oyster moshroom. Ann. Phytopathol. Soc. Jpn. 61:493-502.   DOI
3 Soler-Riveas, C., Jolivet, S., Arpin, N., Oliver J. M., and Wichers, H. J. 1999. Biochemical and physiological aspects of brown blotch disease of Agaricus bisporus. FEMS Microbiology Reviews 23:591-614.
4 Tsuneda, A., Suyama, K., Murakami, S., and Ohira, A. 1995. Occurrence of Pseudomonas tolaasii on fruiting bodies of Lentimula edodes formed Quercus logs. Mycoscience 36:283-288.   DOI   ScienceOn
5 Wells, J. M., Sapers, G. M., Fett, W. F., Butterfield, J. E., Jones, J. B., Bouzar, H., and Miller, F. C. 1996. Postharvest discoloration of the cultivated mushroom Agaricus bisporus caused by Pseudomonas tolaasii, P. ‘reactans’, and P. ‘gingeri’. Phytopathology 86:1098-1104.   DOI
6 Brodey, C. L., Rainey, P. B., Tester, M. and Johnstone, K. 1991. Bacterial blotch disease of the cultivated mushroom is caused by an ion channel forming lipodepsipeptide toxin. Mol. Plant-Microbe Interaction 1:407-411.
7 Grewal, S. I. S., Han, B., and Johnstone, K. 1995. Identification and characterization of a locus which regulates multiple functions in Pseudomonas tolaasii, the cause of brown blotch disease of Agaricus bisporus. J. Bacteriol. 177:4658-4668   DOI
8 Munsch, P., Olivier J., and Houdeau, G. 1991. Experimental control of bacterial blotch by bacteriophages. Science of cultivation edible fungi.Maher(ed) Balkema. Rotterdam. pp. 389-396.
9 Murata, H., Tsukamoto, T., and Shirata, S. 1998. rtp A, a gene encoding a bacterial two-component sensor kinase, determines pathogenic traits of Pseudomonas tolaasii, the causal agent of brown blotch disease of a cultivated mushroom, Pleurotus ostreatus. Mycoscience 39:261-271.   DOI   ScienceOn
10 Nair, N. G., and Fahy, P. C. 1973. Toxin production by Pseudomonas tolaasii Paine. Aust. J. biol. Sci. 26:509-512.   DOI
11 Nutkins, J. C., Mortishire-Smith, R. J., Packman, L. C., Brodey, C. L., Rainey, P. B., Johnstone, K., and Williams, D. H. 1991. Structure determination of tolaasin, an extracellular lipodepsipeptide produced by the mushroom pathogen Pseudomonas tolaasii Paine. J. Am. Chem. Soc. 113:2621-2627.   DOI
12 Rainey, P. B., Brodey, C. L., and Johnstone, K. 1991. Biological properties and spectrum of activity of tolaasin, a lipodepsipeptide toxin produced by the mushroom pathogen Pseudomonas tolaasii Paine. Physiological and Molecular Plant Pathology 39:57-70.   DOI
13 Rainey, P. B., Brodey, C. L., and Johnstone, K. 1993. Identofication of a gene cluster encoding three high-molecular-weight proteins, which is required for synthesis of tolaasin by the mushroom pathogen Pseudomonas tolaasii. Mol. Microbiol. 8:643-652.   DOI   ScienceOn
14 김우재. 2002. 세균성갈색무늬병에 대한 느타리 품종저항성. 농학석사학위논문. 충북대학교.
15 김종완, 김근희, 강희진. 1994. 인공 재배버섯에 질병을 일으키는 Pseudomonas속 병원세균에 관한 연구; 1. 인공 재배버섯의 부패 변성 원인세균에 대하여. 한국식물병리학회지 10:197-210.   과학기술학회마을
16 신관철, 전낙범. 1991. 느타리 세균성갈반병의 병원균 분류 동정 및 생물학적 방제. 농시논문집 34:1-10.
17 이혁인. 1999. 느타리 세균성갈색무늬병균 Pseudomonas tolaasii의 검출 및 동정을 위한 PCR방법 개발. 농학석사학위논문. 충북대학교.
18 조용섭, 박창석, 이순구, 이영근, 임춘근, 차재순, 최용철, 최재을, 허성기, 황인규. 2002. 식물세균병학. 서울대학교출판부. pp. 295-297.
19 주영철, 하태문, 이윤혜, 이한범, 전대훈, 최종인, 김정한, 장명준. 2008. 버섯재배 바로알기. pp. 12-164.
20 진경식, 김정준, 전창성, 이은종. 1994. 버섯 갈변병균 Pseudomonas tolaasii와 Pseudomonas agarici의 분리동정에 있어서 특성차이. 농업논문집 36:343-348
21 차동열, 박정식, 유창현, 김광포, 전창성, 이두원. 1997. 느타리 재배 기술과 재배경영. 농민신문사 p374.