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http://dx.doi.org/10.4489/KJM.2009.37.1.041

Morphological and Physiological Characteristics of Amanita hemibapha subsp. hemibapha (Berk. & Broome) Sacc.  

Sou, Hong-Duck (Department of Forestry, College of Agricultural Life Science, Kyungpook National University)
Hur, Tae-Chul (Department of Forestry, College of Agricultural Life Science, Kyungpook National University)
Joo, Sung-Hyun (Department of Forestry, College of Agricultural Life Science, Kyungpook National University)
Publication Information
The Korean Journal of Mycology / v.37, no.1, 2009 , pp. 41-48 More about this Journal
Abstract
This study was conducted to investigate optimum cultural conditions for Amanita hemibapha isolated in Korea and its hyphae characteristics. Micrographs shows the presence of clamp connection. A. hemibapha grows as mycelial form(M-phase) 2-4 ${\mu}m$ and yeast-like form(Y-phase) 7-8 ${\mu}m$. The fungal spores were broadly elliptical and papillate, 8-11 ${\times}$ 6-9 ${\mu}m$ in size. The nucleotide sequence analysis of the ITS of nuclear ribosomal DNA from sporocarps and in-vitro-grown mycelium supported the fungal species is Amanita hemibapha. A. hemibapha showed sequence similarity in the ITS rDNA with A. caesarea(97.5) and A. jacksonii(98.5%) which are morphologically similar species to A. hemibapha. The optimal pH and temperature for mycelial growth of A. hemibapha were pH 6.0 and $28^{\circ}C$, respectively. The fungal species showed best growth in SYP and GYS medium. A. hemibapha grew well with mannitol and glucose as carbon sources and peptone as a nitrogen source.
Keywords
Ectomycorrhiza; Growth conditions; In vitro culture; Mycelia;
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