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http://dx.doi.org/10.4489/KJM.2008.36.1.093

Expression and Cloning of Microbial Transglutaminase in S. cerevisiae  

Kim, Hyoun-Young (Institute of Basic and Natural Science, Woosuk University)
Oh, Dong-Soon (Department of Pharmaceutical Engineering, Woosuk University)
Kim, Jong-Hwa (Department of Pharmaceutical Engineering, Woosuk University)
Publication Information
The Korean Journal of Mycology / v.36, no.1, 2008 , pp. 93-97 More about this Journal
Abstract
A $Ca^{2+}-independent$ microbial transglutaminase (mTGase) from the actinomycete Streptomyces mobaraensis IFO13819 is a useful enzyme in the food industry. It is consists 406 amino acid residues, which comprised leader and pro region of 75 amino acid residues and the structure region of 331 amino acid residues. Pro and structure gene of TGase were cloned into the yeast shuttle vector pYAEG-TER and then used to transform Saccharomyces cerevisiae 2805. Expression of mTGase in recombinant was confirmed with Northern hybridization and the maximal activity of TGase was shown 26 mU/ml.
Keywords
Heterologus gene expression; Kluyveromyces marxianus; Microbial transglutaminase; Saccharomyces cerevisiae; Streptomyces mobaraensis;
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1 Ikurak, S. R. and Mokito, M. 1992. Use of transglutaminase in quality-improvement and processing of food proteins. Commenta. Agric. Food Chem. 2:389-407
2 Kim, C. H., Kim, H. S. and Kang, Y. J. 1992. The hydrolysis conditions of rapeseed protein by proteinase. J. Korean Soc. Food Nutr. 25:513-518
3 Motoki, M. and Seguro, K. 1998. Transglutaminase and its use for food processing. Food Sci. Technol. 9:204-210   DOI   ScienceOn
4 Motoki, M., Nio, N. and Takinami, K. 1984. Functional properties of food protein polymerized by transglutaminase. Agric. Biol. Chem. 48:1257-1261   DOI
5 Cozzolino, A., Di Pierro, P., Mariniello, L., Sorrentino, A., Masi, P. and Porta, R. 2003. Incorporation of whey proteins into cheese curd by using transglutaminase. Biotechnol. Appl. Biochem. 38:289-295   DOI   ScienceOn
6 Cross, A. J. and Sizer, I. W. 1959. Oxidation of triamine, tyrosine, and related compounds by peroxidase. J. Biol. Chem. 234: 1611-1614
7 Folk, J. E. 1980. Transglutaminase. Annu. Rev. Biochem. 49:517-531   DOI   ScienceOn
8 Kuraishi, C. and Soeda, T. (eds.) American Chemical Society, Washington, DC, USA
9 Ando, H., Adachi, M., Umeda, K., Matsuura, A., Nonaka, M., Uchio, R., Tanaka, H. and Motoki, M. 1989. Purification and characteristics of a novel transglutaminase derived from microorganisms. Agric. Biol. Chem. 53:2613-2617   DOI
10 Anfinsen, L. B. 1973. Principle that govern the folding of protein chains. Science 1811:223-230
11 Tasi, G. J., Lin, S. M. and Jiang, S. T. 1998. Transglutaminase from Streptoverticillium ladakanum and application to minced fish product. J. Food Sci. 61:1234-1238   DOI   ScienceOn
12 Whitaker, J. R. 1997. Enzymatic modification of proteins applicable to foods. pp. 99-155. In: Food proteins improvement through chemical and enzymatic modification. Ed. R. E. Feeny. American Chemical Society, Washington, DC, USA
13 Yasueda, H., Kumazawa, Y. and Motoki, M. 1994. Purification and characterization of a tissue-type transglutaminase from red ses bream (Pagrus Major). Biosci. Biotechnol. Biochem. 58: 2041-2045   DOI   ScienceOn
14 Yokoyama, K., Nio, N. and Kikuchi, Y. 2004. Properties and applications of microbial transglutaminase. Appl. Microbiol. Biotechnol. 64:447-454   DOI
15 Yoo, J.-S., Chun, G.-T. and Jeong, Y.-S. 2003a. The effect of dissolved oxygen on microbial transglutaminase Production by Sterptoverticillium mobaraense. Kor. J. Biotechnol. 18:155-160
16 Motoki, M. and Seguro, K. 1996. Characteristics of microbial transglutaminase and its application on food. Proceedings of the international symposium on recent advances in bioindustry, pp. 79-86
17 Pisamo, J. J., Finlayson, J. S. and Peytron, M. P. 1968. Cross-link in fibrin polymerized by factor XIII $\varepsilon$-(${\gamma}$-glutamyl)lysine. Science 160:892-893   DOI   ScienceOn
18 Sakamoto, H., Yamazaki, K., Kaga, C., Yamamoto, Y., ito, R. and Kurosawa, Y., 1996. Strength enhancement by addition of microbial transglutaminase during chinese noodle processing. Nippon Shokuhin Kagaku Kaishi 43:598-602   DOI   ScienceOn
19 Lou, M. F. 1975. Isolation and identification of L-aspartyl-Llysine and L-$\epsilon$-glutamyl-L-ornithinefrom nomal human urine. Biochemistry 14:3503-3508   DOI   ScienceOn
20 Seota, K. 1997. A study of new protein ingredient by transglutaminase. Shouhing Kougok 12:18-25
21 Kim, H.-S., Lee, D. H., Ryu, E. J., Uhm, T.-B., Yang, M.-S., Kim, J. B. and Chae, K. S. 1999. Expression of the inu2 gene for an endoinulinase of Aspergillus ficumm in Saccharomyces cerevisiae. Biotech. Letters 21:621-623   DOI   ScienceOn
22 Kim, H.-E., Rui, Q. and Chae, K.-S. 2005. Increased production of exoinulinase in Saccharomyces cerevisiae by expressing the Kluyveromyces marxianus INU1 gene under the control of the INU1 promoter. Kor. J. Microbiol. Biotechnol. 15:447-450
23 Kim, H. S. and Kang, Y. J. 1995. Deamination on glutaminyl and asparaginyl residues of protein by Neutrase. Kor. J. Food Sci. Tech. 27:794-798
24 Fork, J. E. and Cole, P. W. 1986. Strucutual requirement of specific substrate for guinea pig liver transglutaminase. J. Biol. Chem. 240:2951-2960
25 Lee, H. G., Chung, M. S. and Choi, Y. J. 1995. A review on application of transglutaminase for animal food products. Kor. J. Sci. 15:252-256
26 Iceson, I. and Apelbaum, A. 1987. Evidence for transglutaminase activity in plant tissue. Plant Physiol. 84:972-974   DOI   ScienceOn
27 Kurth, L. and Rogeners, P. J. 1984. Transglutaminase catalyzed crosslinking of myosin to soya protein, casein, and gluten. J. Food Sci. 49:573-576   DOI
28 Park, E.-H., Shin, Y.-M., Lim, Y.-M., Kwon, T.-H., Kim, D.-H. and Yang, M. S. 2000. Expression of glucose oxidase by using recombinant yeast. J. Biotech. 81:35-44   DOI   ScienceOn
29 Yoo, J.-S., Shin, W.-S., Chun, G.-T., Kim, Y.-S. and Jeong, Y.-S. 2003b. The separation of transglutaminase produced from Streptomyces mobaraensis and its application on model food system. Kor. J. Food Sci. Technol. 35:260-265   과학기술학회마을
30 Yokoyama, K., Nakamura, N., Seguro, K. and Kubota, K. 2000. Overproduction of microbial transglutaminase in Escherichia coli, in vitro refolding, and characterization of the refolded form. Biosci. Biotechnol. Biochem. 64:1263-1270   DOI   ScienceOn