Browse > Article
http://dx.doi.org/10.4489/KJM.2007.35.2.096

Screening of GLA (γ-Linolenic Acid) from Fungi by Gas Chromatography and Mass Spectroscopy  

Kim, Jung-Bong (National Institute of Agricultural Biotechnology, Rural Development Administration)
Kim, Kyung-Hwan (National Institute of Agricultural Biotechnology, Rural Development Administration)
Hong, Seung-Beom (National Institute of Agricultural Biotechnology, Rural Development Administration)
Park, Jong-Sug (National Institute of Agricultural Biotechnology, Rural Development Administration)
Lee, Jong-Yeoul (National Institute of Agricultural Biotechnology, Rural Development Administration)
Kim, Sam-Sun (National Institute of Agricultural Biotechnology, Rural Development Administration)
Bae, Shin-Chul (National Institute of Agricultural Biotechnology, Rural Development Administration)
Cho, Kang-Jin (National Institute of Agricultural Biotechnology, Rural Development Administration)
Lee, Dong-Jin (College of Bio-resources Science, Dankook University)
Publication Information
The Korean Journal of Mycology / v.35, no.2, 2007 , pp. 96-100 More about this Journal
Abstract
In order to select ${\gamma}-Linolenic$ acid (GLA)-producing fungi, a total of forty-four strains of 4 genera such as Phytophthora, Pythium, Mucor and Rhizopus were obtained from Koran Agricultural Culture Collection (KACC) and then analysed by using GC-FID and GC-MS. GLA was detected on 39 fungal strains, and the highest rate of GLA was found as 24.8% of total fatty acids on Mucor hiemalis f. sp. hiemalis KACC 40264. Total GLA content of Zygomycota was comparatively high - Mucor (14.2%) and Rhizopus (14.3%), whereas that of Oomycetes was low - Phytophthora (3.3%) and Pythium (3.0%). Moreover, total fatty acids of the Zygomycota fungi such as Mucor (15.4 mg/100 ml) and Rhizopus (7.1 mg/100 ml) were higher compared with the Oomycetes such as Phytophthora (2.6 mg/100 ml) and Pythium (4.5 mg/100 ml). Thus, two genera such as Mucor and Rhizopus have higher potential as an useful microbial resource. The total fatty acid content varies even within the strains of the same genus e.g. Mucor. M. blumbeus KACC 40935 showed the highest values on productivity (18.2%) of GLA and total fatty acid contents (50.8 mg/100 ml liquid medium).
Keywords
${\gamma}-Linolenic$ acid; Mucor; Phytophthora; Pythium; Rhizopus;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Elena, C., Miroslav, S., Silvia, S. and Flavio, Z. 2001. ${\gamma}$-linolenic acid production by solid-state fermentation of Mucorales strains on cereals. Biores. Tech. 76: 283-286   DOI   ScienceOn
2 Hiruta, O., Kamisaka, Y., Yokochi, T., Futamura, T., Takebe, H. and Satoh, A. et al. 1996. Gamma-linoleic acid production by a low temperature-resistant mutant of Mortierella ramanniana. J. Fermen. Bioeng. 82: 119-123   DOI   ScienceOn
3 Kennedy, M. J., Reader, S. L. and Davies, R. J. 1993. Fatty acid production characteristics of fungi with particular emphasis in gamma-linolenic acid production. Biotech. Bioeng. 42: 625-634   DOI   ScienceOn
4 Saskia, T. and Walter, V. 2005. A gas chromatography/electron ionization-mass spectrometry-selected ion monitoring method for determining the fatty acid pattern in food after formation of fatty acid methyl esters. J. Agric. Food Chem. 53: 8896-8903   DOI   ScienceOn
5 Whipkey, A., Simon, J. E. and Janick, J. 1998. In vivo and in vitro lipid accumulation in Borago officinalis L. J. Am. Oil Chem. Soc. 65: 979-984   DOI
6 Wolf, R. B., Kleiman, R. and England, R. E. 1983. New sources of ${\gamma}$-linolenic acid. J. Am. Oil Chem. Soc. 60:1858-1860   DOI
7 Xian, M., Nie, J., Meng, Q., Liu, J., Zhou, C., Kang, Y. et al. 2003. Production of gamma-linolenic acid by disrupted mycelia of Mortierella isabellina. Lett. Appl. Microbial. 36: 182-185   DOI   ScienceOn
8 Ronald, S. P. 2006. Nutritional intervention with omega-3 fatty acids enhances tumor response to anti-neoplastic agents. Chem. Biol. Interact. 162: 89-105   DOI   ScienceOn
9 Tan, C. K. and Johns, M. R. 1991. Fatty acid production by heterotrophic Chlorolla sacchanophilas. Hydrobiologia 215: 13-19   DOI
10 Hinman, J. W. 1972. Prostagrandins. Annu. Rev. Biochem. 41: 161-178   DOI   ScienceOn
11 Owen, P. W. and Ajay, S. 2005. Omega-3/6 fatty acids: Alternative sources of production. Process Biochem. 40: 3627-3652   DOI   ScienceOn
12 Jessup, W. and Fowler, M. W. 1976. Interrelationship between carbohydrate metabolism and nitrogen assimilation in cultured plant cells. I. Effect of glutamate and nitrate as alternative nitrogen sources on cell growth. Planta 132: 119-125   DOI   ScienceOn
13 Kim, J. B., Kim, Y. H., Lee, C. H., Hwang, Y. S. and Park, R. D. 1995. Screening of ${\gamma}$-linolenic acid resources and fatty acid composition in Korean native medicinal plants. Kor. J. Med. Crop. Science 3: 107-110
14 Kim, Y. H. and Janick, J. 1991. Abscisic acid and proline improve desiccation tolerance and increase fatty acid content of celery somatic embryos. Plant Cell Tissue Organ Culture 24: 83-89   DOI
15 Metcalfe, L. D., Schmitz, A. A. and Pelka, J. R. 1966. Rapid preparation of fatty acid esters from lipids for gas chromatographic analysis. Anal. Chem. 38: 514-515   DOI
16 Bayley, J. M., King, J. and Gamborg, O. L. 1972. The effect of the source of inorganic nitrogen on growth and enzymes of nitrogen assimilation in soybean and wheat cells in suspension cultures. Planta 105: 15-20   DOI   ScienceOn
17 Kim, J. B., Kim, K. H., Hwang, S. K., Kim, Y. H., Cho, K. J., Hwang, Y. S. and Park, R. D. 2001. The Composition of useful medium chain fatty acid in eight plant species. J Kor. Soc. Agric. Chem. Biotechnol. 44: 20-23   과학기술학회마을
18 Certik, M., Balteszova, L. and Sajbidor, J. 1977. Lipid formation and gamma-linolenic acid production by Mucorales fungi grown on sunflower oil. Lett. Appl. Microbiol. 25: 101-105   DOI   ScienceOn