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http://dx.doi.org/10.4489/KJM.2006.34.2.092

Antimicrobial Activity of the Coriolus versicolor Liquid Culture Extracts Against Antibiotic Resistant Bacteria and Purification of Active Substance  

Lee, Jung-Sun (Department of Biotechnology & The Biomaterials Engineering Center, Catholic University of Korea)
Kim, Taeg (Department of Life Science, College of Natural Science, Cheju National University)
Lee, Yoon-Hi (Department of Biotechnology & The Biomaterials Engineering Center, Catholic University of Korea)
Jin, Cheng-Min (Department of Biotechnology & The Biomaterials Engineering Center, Catholic University of Korea)
Kim, Hyun-Guell (Department of Biotechnology & The Biomaterials Engineering Center, Catholic University of Korea)
Kim, Woo-Jung (Department of Biotechnology & The Biomaterials Engineering Center, Catholic University of Korea)
Oh, Duek-Chul (Department of Life Science, College of Natural Science, Cheju National University)
Park, Yong-Il (Department of Biotechnology & The Biomaterials Engineering Center, Catholic University of Korea)
Publication Information
The Korean Journal of Mycology / v.34, no.2, 2006 , pp. 92-97 More about this Journal
Abstract
The liquid culture extract of Coriolus versicolor was prepared by directly boiling the whole culture broth 7 days after incubation in 12% citrus extract medium. After removal of mycelial debris through filtration, this extract was further extracted with equal volume of ethyl acetate (1 : 1, v/v). The ethyl acetate extracts showed significant antibacterial activities against Stapylococcus aureus CCARM3230 and Psudomonas aeruginosa CCARM2171, which are resistant to several antibiotics. The most active fraction was eluted from a silica gel column with a mixture of dichloromethane and methanol (9 : 1, v/v) and the purity of this active substance was confirmed by HPLC analysis. The results suggest that the purified active substance could be a good source for the development of a new antimicrobial agent, especially for the treatment of antibiotic resistant bacteria.
Keywords
Antibacterial activity; Antibiotic resistant bacteria; Coriolus versicolor; Ethylacetate extract;
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Times Cited By KSCI : 2  (Citation Analysis)
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