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http://dx.doi.org/10.4489/KJM.2006.34.2.112

Selection of Lecanicillium Strains for Aphid (Myzus persicae) Control  

Jung, Hye-Sook (Department of Bioscience & Biotechnology, The University of Suwon)
Lee, Hyang-Burm (Division of Applied Bioscience and Biotechnology, Chonnam National University)
Kim, Keun (Department of Bioscience & Biotechnology, The University of Suwon)
Lee, Eun-Young (Department of Environmental Engineering, The University of Suwon)
Publication Information
The Korean Journal of Mycology / v.34, no.2, 2006 , pp. 112-118 More about this Journal
Abstract
To select efficient entomopathogenic fungal strains of Lecanicillium for the biocontrol of aphid, Myzus persicae, conidial suspension ($1{\times}10\;conidia/ml$) was sprayed onto a detached Chinese cabbage leaf in a petri dish with a dampened filter paper that had 20 nymphs of aphid. Lecancillium strain 4078 and 6543 were the best strains for the biocontrol of aphid at high temperature of $30^{\circ}C$ and low relative humidity (RH) of 85%, respectively. The cumulative mortality of strain 4078 at $30^{\circ}C$ after 3 days was 100% and that of strain 6543 was 90% at 85% RH after 5 days. Strain 4078 also exhibited almost 100% germination ratio of conidia and high rate of mycelial growth at the broad temperature-range of $15{\sim}25^{\circ}C$. The strain 4078 and 6543 were all identified as Lecanicillium species based on the DNA sequences (accession no.: EF026004 and EF026005, respectively) of the ITS regions of the fungi. Excellent production of aerial conidia of strain 6543 was accomplished by using steamed polished rice as the solid culture medium.
Keywords
Aphids; Biocontrol; Germination; Lecancillium; Production of conidia;
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