Browse > Article
http://dx.doi.org/10.4489/KJM.2005.33.2.069

Purification and Characterization of Fibrinolytic Enzyme from Lepista nuda  

Kim, Jun-Ho (Department of Chemistry, Sangji University)
Publication Information
The Korean Journal of Mycology / v.33, no.2, 2005 , pp. 69-74 More about this Journal
Abstract
Fibrinolytic enzyme has been isolated and purified from the edible mushroom, Lepista nuda. The apparent molecular mass of purified enzyme was estimated to be 34 KDa by SDS-polyacrylamide gel electrophoresis. The N-terminal amino acid sequence of the enzyme was Tyr-Pro-Ser-Pro-Ser-His-Gln-Thr-Ala-Val-Asn-Ala-Ile-Ile-X. It has a pH optimum at $7.0.{\sim}9.5$, suggesting that the purified enzyme is an alkaline protease. It shows the maximum fibrinolytic activity at $55^{\circ}C$. The fibrinolytic activity was inhibited by phenylmethylsulfonyl fluoride, indicating that the purified enzyme is a serine protease. The activity of the purified enzyme was totally inhibited by $Hg^{2+}$.
Keywords
Fibrin plate assay; Fibrinolytic enzyme; Lepista nuda;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Kim, J. H. and Kim, Y. S. 2001. Characterization of a metalloenzyme from a wild mushroom, Tricholoma saponaceum, Biosci. Biotech. Biochem. 65(2): 356-362   DOI   ScienceOn
2 Kim, Y. T., Kim, W. K. and Oh, H. S. 1996. Purification and characterization of a fibrinolytic enzyme produced from Bacillus sp, strain CK 11-4 screened from ChungkookJang. Appl. Environ. Microbiol. 62: 2482-2488
3 Kubo, M., Tatsuda, H., Nogarni, M., Arichi, S. and Takahashi, T. 1983: Studies on the Ganoderma lucidum (IV), effects on the disseminated intravascular coagulation. Yakugaku Zasshi 103: 871-877   DOI
4 Lowry, O. H., Rosenbrough, N, J. and Randall, A. J. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265-275
5 Mihara H., Nakajima, N. and Surni, H, 1993, Characterization of potent fibrinolytic enzymes in earthworm, Lumbricus rubellus. Biosci. Biotech. Biochem. 57(10): 1730
6 Nonaka, T., Dohmae, N., Hashimoto, Y. and Takio, K. 1997. Amino acid sequences of metaltoendopeptidases specific for Acyl-Lysine bonds from Grifola frondosa and Pleurotus ostreatus fruiting bodies, J. Biol. Chem. 272: 30032-30039   DOI   ScienceOn
7 Park, Y. D., Kim, J. W., Min, B. G., Seo, J. W. and Jeong, J. M. 1998. Rapid purification and biochemical characteristics of Lumbrokinase III from earthworm for use as a fibrinolytic agent. Biotechnol. Lett. 20(2): 169-172   DOI   ScienceOn
8 김길영. 1998, 임상지혈혈전학, 군지출판사. 33pp
9 김병각, 김진숙, 최응칠, 김혜령, 이종길, 이정옥, 정경수, 심미자. 1983 한국산 고등 균류의 성분 연구(제 37보). 한국균학회지 11: 151-157   과학기술학회마을
10 박상신, 이갑득, 민태진. 1995. 버섯 중 항균물질의 검색 및 개발에 관한 연구. 한국균학회지 23(1): 28-36   과학기술학회마을
11 안덕균. 1992, 한국산 약용 균류, 한국균학회지 20: 154-166   과학기술학회마을
12 Chung, K. H. and Kim, D. S. 1992. Fibrinolytic and cogulation activities of Korean snake venoms. Kor. Biochem. J. 25: 696-701
13 Gavrilova, V. P. and Falina, N. N. 1975. Proteolytic enzyme isolated from a fungus, Flammulina velutipes (Fr). Sing. Mikol. Fitopatol. 9; 431-433
14 Haverkate, F. and Traas D. W. 1974. Dose-response curves in the fibrin plate assay. fibrinolytic activity of protease. Thromb. Haemost. 32; 356-365
15 Kabir, Y. and Kimura, S. 1989. Dietary mushrooms reduce blood pressure in spontaneously hypertensive rats (SHR). J. Nutr. Sci. Vitaminol. 35: 91-94   DOI
16 Kim, J. H. 2002. The Screening test of wild mushroom extracts for fibrinolytic activitiy, Kor. J. Biomed. Lab. Sci. 8(3): 173-177
17 Daka, M. D. and Semba, C. P. 1995. Thrombolytic therapy in venous occlusive disease. J Vase lnterv Radiol, 6:(suppl) 73-77   DOI   ScienceOn
18 Shin, H. H. and Choi, H. S. 1998. Purification and partial characterization of a Metalloprotease in Flammulina velutipes. J. Microbiol. 36: 20-25
19 Stavinoha, W. B. and Satsangi, N. 1997. The 7th international symposium on the Ganoderma Lucidum. Seoul, Korea, 11 June. pp. 5-17
20 김승호. 1998. 된장의 가능성에 대한 새로운 연구방향-혈전용해 능에 관하여. 한국콩연구회지 15: 8-15
21 Kim, H. K., Kim, G T., Kim, D. K., Choi, W. A., Park, S. H., Jeong, Y. K. and Kong, I. S. 1997. Purification and characterization of a novel fibrinolytic enzyme from Bacillus sp. KA38 originated from fermented fish. J. of Fermentation and Bioengineering. 84(4): 307-312   DOI   ScienceOn
22 김준호, 이호용, 유관희, 김양선, 석순자, 김양섭. 1998. 치악산 버섯추출물로부터 Fibrin 분해활성의 검색, 한국균학회지 26: 589-593   과학기술학회마을
23 Kim, J. H. 2002. Purification and characterization of fibrinolytic enzymes from Tricholoma sejunctum, Kor. J Biomed. Lab. Sci. 8(3); 173-177
24 Kim, J. H. and Kim, Y. S. 1999. A fibrinolytic metaJloprotease from the fruiting bodies of an edible mushroom, Armillariella mellea. Biosci. Biotech. Biochem. 63(12): 2130-2136   DOI   ScienceOn