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http://dx.doi.org/10.4489/KJM.2003.31.3.187

Optimal Condition for Mycelial Production of Armillaria mellea  

Kim, Myung-Kon (Department of Food Resources and Environment, Iksan National College)
Choi, Han-Seok (Department of Food Science and Technology, Chonbuk National University)
Park, Hyo-Suk (Department of Agricultural Chemistry, Wonkwang University)
Kim, Sung-Jun (Department of Genetic Engineering, Chosun University)
Publication Information
The Korean Journal of Mycology / v.31, no.3, 2003 , pp. 187-191 More about this Journal
Abstract
Armillaria mellea, honey mushroom is well known as a symbiotic fungus with Gastodia elata, The mycelial yields of the fungus were compared when cultured with various broth media. The highest yield of cell mass, 2.31 g dry weight/50mL, was obtained on germinated-malt extract broth (GMEB). The optimal broth concentration which was measured hand refractometer for mycelium production was $15\;Brix^{\circ}$. The optimal conditions estimated with response surface methodology under temperature, pH and incubation period were $25.9^{\circ}C$, pH 5.72, 15.22 days, respectively, on GMEB having $15\;Brix^{\circ}$ concentration for mycelial production of A. mellea.
Keywords
Armillaria mellea; Germinated-malt extract medium; Optimal condition; Response surface methodology;
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